A New Method for Determination of ABO Blood Groups using Nitrocellulose

硝化纤维测定 ABO 血型的新方法

• 批准号: 61870031
• 负责人: SHIKATA Ichiro
• 金额: $ 2.05万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61870031/
• 关键词: Nirocellulose; ABO-blood-group system; ABC-method; Fingerprint; 血痕

(1) ABO grouping was carried out on fingerprints lifted onto nitrocellulose membrane using the ABC method or mixed coll agglutination reaction. Specimens of fingerprints were obtained by gentle pressing of fingertips onto nitrocellulose membrenes by lifting the fingerprints experimentally prepared on glass slides onto nitrocellulose sembrenes .1:Mixed cell agglutination reaction was performed by incubating the membranes with anti-A, anti-B serum or anti-HUlec lectin followed by washing and further incubation with A.B or O type erythrocytes. This technic is relatively simpler and clearer rather than the conventional method using sticking film plate and is very useful in the practice of blood lyping of fingerprints in the forensic science field. 2:Above specimens were sensitized with anti-A and anti-B sera os varying dilutions. The specifically absorbed antibodies were visualized by the ABC method. In result. group A and AB fingerprints showed positive staning for anti-A whercas group B … More and O fingerprints ahowed no positive staining. in a similar manner, group B and AB fingerprints showed positive staining for anti-B. whereas group A and O fingerprints showed no positive staining. Any positive staining with appreciable internsity could not be observed in this experiment.(2) We evaluated the advantage of using nitrocellulose beads as immunoadsorbents for ABH blood groups. The blood group substances in hemolyzed and highly diluted blood specimens were absorbed on the boads. after which the blood type was determined either by mixed cell agglutination or by the absorption-elusion technique. The latter was found to be more sensitive than the former. An optimal absorption of the ABH substances was obtained when we incubated 1 mg nitrocellulose beads with 10 ml of the blood samples for 1 hour at 25゜C. A 100000-fold diluted antigen of the A or B type or a 50000-fold diluted antigen of the H type was detected under these conditions. No procedures of extraction. purification. or concentrations of the antigen were needel. It is suggested that the new methods described above will find wider application in the practice of medico-legal examination of trace evidence. Less

(1)采用ABC法或混合胶凝集反应对硝化纤维膜上的指纹进行ABO分组。通过将在载玻片上实验制备的指纹提升到硝化纤维素膜上，用指尖轻轻按压硝化纤维素膜来获得指纹样本。1：通过将膜与抗A、抗B血清或抗HUlec凝集素一起孵育，然后洗涤并进一步与A.B或O型一起孵育来进行混合细胞凝集反应 红细胞。该技术比传统的贴膜板方法相对简单、清晰，在法医学领域的指纹血检实践中非常有用。 2：以上标本用不同稀释度的抗A和抗B血清致敏。通过ABC法使特异性吸收的抗体可视化。结果。 A 组和 AB 指纹显示抗 A 阳性染色，而 B 组和 O 指纹无阳性染色。以类似的方式，B 组和 AB 指纹显示抗 B 阳性染色。而A组和O组指纹未显示阳性染色。本实验中未观察到任何具有明显强度的阳性染色。(2)我们评估了使用硝化纤维素珠作为ABH血型免疫吸附剂的优势。溶血和高度稀释的血液标本中的血型物质被吸附在板上。然后通过混合细胞凝集或吸收洗脱技术确定血型。发现后者比前者更敏感。当我们将 1 mg 硝酸纤维素珠与 10 ml 血液样本在 25°C 下孵育 1 小时时，获得了 ABH 物质的最佳吸收。在这些条件下检测A或B型抗原的100000倍稀释或H型抗原的50000倍稀释。没有提取程序。纯化。或抗原的浓度是需要的。相信上述新方法将在微量证据的法医学检验实践中得到更广泛的应用。较少的

项目成果

藤谷登: 科学警察研究所報告. 39. 208-211 (1986)

Noboru Fujitani：科学警察研究所的报告 39. 208-211 (1986)。

Noboru Fujitani.: "ABO Grouping of Fingerprints Lifted onto Nitrocellulose Sheets by the Use of ABC Method" Reports of the National Research Institute of Police Science. 39. 208-211 (1986)

Noboru Fujitani.：“使用 ABC 方法将指纹提升到硝化纤维片上的 ABO 分组”国家警察科学研究所的报告。

藤谷登: 日本法医学雑誌. 42. 318-323 (1988)

藤谷登：日本法医学杂志 42. 318-323 (1988)。

Noboru Fujitani.: "Determination of ABO Blood Groups in Highly Diluted Blood by a New Method Using Nitrocellulose Beads" The Japanese Journal of Legal Medicine. 42. 318-323 (1988)

Noboru Fujitani.：“使用硝化纤维素珠的新方法测定高度稀释血液中的 ABO 血型”《日本法医学杂志》。