Studies on the Landsteeiner Phenomenon of the ABO Blood Group System in Nature

考虑到去年的研究结果，我们进行了我们的研究，得到了以下结果：我们检查了植物、土壤细菌、鱼类和无脊椎动物是否有像人类一样的兰德斯坦纳现象。这是首次发现Landsteiner现象。Landsteiner现象存在于植物Clerodendrom trichctomus的叶、花和茎中，存在于土壤细菌Prokopoa nagai ensis中，存在于鱼类鲑鱼和鳟鱼的卵中，存在于无脊椎动物Helix pomatia的蛋白区分泌物中。这些发现对于从分子生物学的角度阐明生命的进化是非常重要的。建立了Le^a-转移酶、xiv -反式聚焦酶、H- - d -半乳糖转移酶和H- - n -乙酰- d -半乳糖转移酶活性的定量测定方法。为了检验多年来血型物质稳定性的变化，我们利用90年前罗伯特·科赫寄给他的弟子弗洛施教授的信的信封上的唾液，并从罗伯特·科赫研究所和奥托·普罗科普教授（柏林洪堡大学法医学研究所）获得，我们确定了ABO和刘易斯血型，它们具有与ABO血型相似的化学结构。经绝对度检验，所得结果为0，Le （a+, b-）。因此，很明显，血型物质分泌到唾液中是相当稳定的。我们建立了利用某段恶性肿瘤组织在玻片上转化血型物质（合成和分解）的方法。利用微生物半乳糖转移酶，阐明了感染区组织中血型物质转化的可能性，从而阐明了感染区组织中血型物质转化的机制。我们尝试了在“血液遗传学领域”备受关注的细胞DNA分型，并进行了检验

We carried out our study, considering results of our last-year study, and obtained following results.1. We examined if plants, soil bacteria, fishes and invertebrates have Landsteiner phenomenon like human case. It was first time for us to find Landsteiner phenomenon is seen in leaves, flowers and stems of the Clerodendrom trichctomus as a plant and in the Prokopoa nagai ensis as soil bacterum and in eggs of salmons and trouts as fishes and in secretion from albumenglands of the Helix pomatia as a invertebrates. Those discoveries are very important to make evolution of life clear in terms of molecular biology.2. We established quantitative method for Le^a-transferase, XIV-transfucosidase, H- -D-galactose-transferase and H- -N-Acetyl-D-galactosaminetransferase activity.3. To examine change of stability of blood group substance which passed many years, using saliva on the envelope of the letter which Robert Koch sent to his disciple Prof. Flosch about 90 years ago and which could get from Robert Koch institute with Prof. Otto Prokop (Institute of Forensic Medicine,Humboldt-University, Berlin), we identified ABO and Lewis blood group which has chemical structure which is similar to ABO blood group. The result was obtained as O, Le (a+, b-) with absolubtion test. And so it could be clear that blood group substance secretes into saliva is quite stable.4. We established the way to transform blood group substance (synthesis and decomposition) on slide-glasses, using a segment of certain tissue with malignant tumor.5. Using galactosyltransferase from microorganisms, we made the mechanism of transformation of blood group substance in the tissues of infection area by elucidating that transform of blood group substance in tissues in possible.6. We tried DNA typing in cells which is paid attention ti in "Hemogenetics field" and we examine