Studies on the Landsteeiner Phenomenon of the ABO Blood Group System in Nature

自然界ABO血型系统Landsteiner现象的研究

• 批准号: 01490020
• 负责人: NAGAI Tatsuo
• 金额: $ 0.96万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01490020/
• 关键词: ABO blood group; Landsteiner's phenomenon; Stability of blood group substance; Blood group of bacteria; Molecular biology of blood group substance; Biogenetics of blood group substance; Biosynthesis and enzyme for blood group substance; Marker of t

We carried out our study, considering results of our last-year study, and obtained following results.1. We examined if plants, soil bacteria, fishes and invertebrates have Landsteiner phenomenon like human case. It was first time for us to find Landsteiner phenomenon is seen in leaves, flowers and stems of the Clerodendrom trichctomus as a plant and in the Prokopoa nagai ensis as soil bacterum and in eggs of salmons and trouts as fishes and in secretion from albumenglands of the Helix pomatia as a invertebrates. Those discoveries are very important to make evolution of life clear in terms of molecular biology.2. We established quantitative method for Le^a-transferase, XIV-transfucosidase, H- -D-galactose-transferase and H- -N-Acetyl-D-galactosaminetransferase activity.3. To examine change of stability of blood group substance which passed many years, using saliva on the envelope of the letter which Robert Koch sent to his disciple Prof. Flosch about 90 years ago and which could get from Robert Koch institute with Prof. Otto Prokop (Institute of Forensic Medicine,Humboldt-University, Berlin), we identified ABO and Lewis blood group which has chemical structure which is similar to ABO blood group. The result was obtained as O, Le (a+, b-) with absolubtion test. And so it could be clear that blood group substance secretes into saliva is quite stable.4. We established the way to transform blood group substance (synthesis and decomposition) on slide-glasses, using a segment of certain tissue with malignant tumor.5. Using galactosyltransferase from microorganisms, we made the mechanism of transformation of blood group substance in the tissues of infection area by elucidating that transform of blood group substance in tissues in possible.6. We tried DNA typing in cells which is paid attention ti in "Hemogenetics field" and we examine

我们进行了我们的研究，考虑到我们去年的研究结果，并获得了以下结果。我们研究了植物、土壤细菌、鱼类和无脊椎动物是否像人类一样具有Landsteiner现象。首次在植物毛盖大青的叶、花和茎中发现了Landsteiner现象，在土壤微生物长井原早熟禾中发现了Landsteiner现象，在鱼类鲑鱼和鳟鱼卵中发现了Landsteiner现象，在无脊椎动物鱼的蛋白质分泌物中发现了Landsteiner现象。这些发现对于从分子生物学的角度阐明生命的进化是非常重要的.建立了Le-α-转移酶、XIV-岩藻糖苷转移酶、H-D-半乳糖转移酶和H-N-乙酰-D-半乳糖胺转移酶活性的定量方法.为了检测血型物质经过多年的稳定性变化，我们使用罗伯特·科赫在大约90年前寄给他的弟子弗洛施教授的信的信封上的唾液，该信封可以从罗伯特·科赫与奥托·普罗科普教授的研究所（柏林洪堡大学法医研究所）获得，我们鉴定了ABO和化学结构与ABO血型相似的刘易斯血型。用绝对溶解法测定结果为O，Le（a+，B-）。由此可见，血型物质分泌到唾液中是相当稳定的.建立了在玻片上进行血型物质转化（合成和分解）的方法，并以恶性肿瘤组织切片为实验材料.利用微生物来源的半乳糖基转移酶，阐明了血型物质在组织中转化的可能性，从而阐明了血型物质在疫区组织中转化的机理.我们尝试了“血液遗传学领域”关注的细胞DNA分型，并进行了检验

项目成果

Nagai,Tatsuo: "Study on the Landsteiner's model of the ABO blood grouping in the microbiological field" Kriminalistik und forens.Wiss.79,80. 97-102 (1990)

Nagai, Tatsuo：“微生物领域 ABO 血型的 Landsteiner 模型研究”Kriminalistik und forens.Wiss.79,80。

Riebeta, O., Kammerbauer, C., Roewer, L., Steimle, V., Andreas, A., Albert, E., Nagai, T., Epplen, JT.: "Hypervariability of intronic simple (gt) n (ga) m repeats in HLA-DRBl genes" Immunogenetics. 32. 110-116 (1990)

Riebeta, O.、Kammerbauer, C.、Roewer, L.、Steimle, V.、Andreas, A.、Albert, E.、Nagai, T.、Epplen, JT.：“内含子简单 (gt) n 的超变性（

Nagai, T., Sugita, Y., Kurihara, M., Ikeda, T., Rose, M.: "Human monocyte antigen (HMA system) in a random sample of Japan" Expl Clin Immunogenet. 7.

Nagai, T.、Sugita, Y.、Kurihara, M.、Ikeda, T.、Rose, M.：“日本随机样本中的人类单核细胞抗原（HMA 系统）”Expl Clin Nutrition。

Rieβ,Olaf: "Hypervariability of infronic simple (gt)n(gt)m repeats in HLAーDR B genes" Immunogenetics. 32. 110-116 (1990)

Rieβ，Olaf：“HLA-DR B 基因中 infronic 简单 (gt)n(gt)m 重复序列的超变异性”免疫遗传学 32. 110-116 (1990)

Nagai,T.and Imamiya,S.: "A quantitative evaluation of ABO gene product" The Kitasato Archives of Experimental Medicine.

Nagai,T. 和 Imamiya,S.：“ABO 基因产物的定量评估”北里实验医学档案馆。