Development and automation of enzyme immunoassay of prostanoids

为了研究前列腺素类化合物的体内代谢，我们建立了一种灵敏、特异的酶联免疫分析方法，并对尿和血中前列腺素类化合物的测定条件进行了研究。11-去氢血栓烷B_2的代谢半衰期比血栓烷B_2本身长，是一个可靠的内源性血栓烷合成参数。该抗体与血栓素B_2几乎无交叉反应，可用于11-脱氢血栓素的放射免疫测定。该抗体还用于从人尿液和血液中免疫亲和提取和纯化11-脱氢血栓烷。对免疫亲和纯化的材料进行放射免疫测定，并通过气相色谱-质谱法确认测定的有效性。

For the study on the in vivo metabolism of bioactive prostanoids we developed an enzyme-linked immunoassay as a sensitive and specific assay method, and investigated the assay conditions of these prostaglandins in urine and blood. We raised a monoclonal antibody against 11-dehydrothromboxane B_2, which has a longer metabolic half-life than thromboxane B_2 itself and is considered as a reliable parameter of the endogenous thromboxane synthesis. The antibody hardly cross-reacted with thromboxane B_2, and used for radioimmunoassay of 11-dehydrothromboxane. The antibody was also utilized for immunoaffinity extraction and purification of 11-dehydrothromboxane from human urine and blood. The immunoaffinity-purified material was subjected to radioimmunoassay, and the validity of the assay was confirmed by gas chromatography-mass spectrometry.