Intra-cellular manipulation of human retroviruses by NOS

NOS 对人类逆转录病毒的细胞内操作

• 批准号: 63870022
• 负责人: HATANAKA Masakazu
• 金额: $ 9.22万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63870022/
• 关键词: NOS; Human retrovirus; HTLV-I; HIV-I; Nucleolar targeting; Messenger RNA; Intra-cellular manipulation; HIV-I; HIV-1; メッセンジャーRNA

We discovered the nucleolar targeting signal (NOS) in human retroviruses, HTLV-I and HIV-I. The NOS consists of peptides with 9-19 amino acid residues, of which the existence leads proteins to accumulate in the nucleoli of cells. The loss of the signal function coincides with the loss of regulatory functions of human retroviruses. such intracellular manipulation of human retroviruses by NOS may provide a new method to prevent the onset of human retroviral diseases.

我们在人类逆转录病毒HTLV-I和HIV-I中发现了核仁靶向信号（NOS）。NOS由含有9-19个氨基酸残基的肽组成，其存在导致蛋白质在细胞核仁中积累。信号功能的丧失与人类逆转录病毒调控功能的丧失是一致的。NOS对人类逆转录病毒的细胞内操纵可能为预防人类逆转录病毒疾病的发生提供一种新的方法。

项目成果

Zhou, Z., et al.: "Expression of post-transcription regulatory gene of HTLV-I, rex, in Escherichia coli" Virus Genes, 3, 153-158, 1989.

Zhou, Z., et al.:“HTLV-I, rex, in Escherichia coli 的转录后调控基因的表达”Virus Genes, 3, 153-158, 1989。

Kubota,S.,et al.: "Functional similarity of HIV-1 rev and HTLV-1 rex proteins:Identification of a new nucleolar-targeting signal in rev protein" BBRC. 162. 963-970 (1989)

Kubota,S.,et al.：“HIV-1 rev 和 HTLV-1 rex 蛋白的功能相似性：rev 蛋白中新核仁靶向信号的识别”BBRC。

Zhou,Z.,et al.: "Expression of post-transcription regulatory gene of HTLV-1,rex,in Escherichia coli" Virus Genes. 3. 153-158 (1989)

Zhou，Z.，et al.：“HTLV-1，rex，在大肠杆菌中的转录后调控基因的表达”病毒基因。

Kubota,S.,et al.: "Functional similarity of HIV-I rev and HTLV-I rex proteins:Identification of a new nucleolar-targeting signal in rev protein" BBRC. 162. 963-970 (1989)

Kubota,S.,et al.：“HIV-1 rev 和 HTLV-1 rex 蛋白的功能相似性：rev 蛋白中新核仁靶向信号的鉴定”BBRC。

Endo,S.,et al.: "A region of basic amino-acid cluster in HIV-1 Tat protein is essential for trans-acting activity and nucleolar lacalization" Virus Genes. 3. 99-110 (1989)

Endo,S.,et al.：“HIV-1 Tat 蛋白中的碱性氨基酸簇区域对于反式作用和核仁拉卡尔化至关重要”病毒基因。