Nucleolar function on formation, stability and translation of messenger RNA

核仁对信使 RNA 形成、稳定性和翻译的功能

• 批准号: 63440024
• 负责人: HATANAKA Masakazu
• 金额: $ 19.97万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63440024/
• 关键词: mRNA; RNA transport; human retrovirus; nucleolar targeting signal; RNA stability; splicing; HTLV-I; HIV-I; メッセンジャーRNA; 翻訳; 核小体; ヒトレトロウィルス; タンパク

The Rex protein is localized in the nucleoli of cells infected by HTLV-I, a human retrovirus causing adult T-cell leukemia. We studied roles of Rex protein in the nucleus and its effect on the formation, stability, transport and translation of messenger RNAS. The Rex protein without the nucleolar targeting signal (NOS) failed to form the unspliced gag-pro-pol messenger RNA of HTLV-I, indicating that the NOS is required for transporting certain type of messenger RNAs from nucleus to cytoplasm. Furthermore, another human retrovirus causing AIDS, HIV-1 contains the NOS in two regulatory gene products, Tat and Rev. We have found that the NOS of Tat and Rev proteins is essential for their functions.

雷克斯蛋白定位于HTLV-1感染细胞的核仁中，HTLV-1是一种引起成人T细胞白血病的人类逆转录病毒。我们研究了雷克斯蛋白在细胞核中的作用及其对信使RNA的形成、稳定性、转运和翻译的影响。没有核仁靶向信号（NOS）的雷克斯蛋白不能形成HTLV-Ⅰ的未剪接的gag-pro-pol信使RNA，表明NOS是将某些类型的信使RNA从细胞核转运到细胞质所必需的。此外，另一种引起艾滋病的人类逆转录病毒HIV-1在两种调节基因产物达特和Rev中含有NOS。我们发现达特和Rev蛋白的NOS对其功能至关重要。

项目成果

Kubota,S.,et al.: "Effects of chimeric mutants of human immunodeficiency virus type I Rev and human T-cell leukemia virus type I Rex on nucleolar targeting signals" J.Virol.65. 2452-2456 (1991)

Kubota,S.,et al.：“人类免疫缺陷病毒 I 型 Rev 和人类 T 细胞白血病病毒 I 型 Rex 的嵌合突变体对核仁靶向信号的影响”J.Virol.65。

Hatanaka, M.: "Discovery of the nucleolar targetin signal" BioEssays. 12. 143-148 (1990)

Hatanaka, M.：“核仁靶向信号的发现”BioEssays。

Hatanka,M.: "Genetic Structure and Regulation of HIV" Raven Press,Ltd.New York, 537 (1991)

Hatanka,M.：“HIV 的遗传结构和调控”Raven Press,Ltd.New York，537 (1991)

White,K.N.,et al.: "The nucleolar localisation of the HTLV-I protein p27rex is important for stabilisation of IL-2 receptor α subunit mRNA by p27rex" Biochem.Biophys.Res.Commun.175. 98-103 (1991)

White, K.N. 等人：“HTLV-I 蛋白 p27rex 的核仁定位对于 p27rex 稳定 IL-2 受体 α 亚基 mRNA 很重要”Biochem.Biophys.Res.Commun.175 (1991)。

Kubota, S.: "Functional conversion from HIV-1 Rev to HTLV-1 Rex by mutation" Biochem. Biophys. Res. Commun.178. 1226-1232 (1991)

Kubota, S.：“通过突变从 HIV-1 Rev 到 HTLV-1 Rex 的功能转换”Biochem。