Plant biotic interactions under disturbance in the salt marsh

盐沼干扰下植物生物相互作用

• 批准号: 403673368
• 负责人: Professor Dr. Dirk Albach
• 金额: --
• 依托单位: Arbeitsgruppe Biodiversität und Evolution der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/403673368?language=en
• 关键词: Plant; biotic; interactions; under; disturbance

Plant interactions with microorganisms often provide essential survivalbenefits to plants, especially under stress and disturbance. However,these interactions can also be detrimental to one partner(parasitism/pathogenicity). The factors that influence the outcome ofthe interaction are poorly understood. However, they are important forour understanding of ecosystem changes during disturbances such asextreme weather events related to climate change. In this subproject,we aim to investigate these interactions using a metatranscriptomic approach. This will involve examining in parallel expressed genes ofplant and endophytic microorganisms. These allow conclusions to bedrawn about the function of the microorganisms, especially whencomparing gene expression under different environmental conditions(here hypersalinity). In addition to the trophic interactions of plant andmicroorganisms, the interaction with invertebrates (herbivory) will alsobe investigated and the food web of the salt marsh will inferred inmore detail with the help of a barcode library and an eDNA dataset.The barcode library is necessary because many plants in the saltmarsh show intraspecific genotypic variation or closely relatedspecies and their hybrids co-occur. The importance of such variationto the species composition of a dynamic ecosystem is the secondfocus of the proposal.

植物与微生物的相互作用通常为植物提供必要的生存益处，特别是在胁迫和干扰下。然而，这些相互作用也可能对一方有害（寄生性/致病性）。影响相互作用结果的因素知之甚少。然而，它们对于我们理解与气候变化有关的极端天气事件等干扰期间的生态系统变化非常重要。在这个子项目中，我们的目标是使用元转录组学的方法来研究这些相互作用。这将涉及检查植物和内生微生物的平行表达基因。这使得我们可以得出关于微生物功能的结论，特别是当比较不同环境条件下的基因表达时（这里是高盐）。除了植物和微生物之间的营养相互作用外，还将研究与无脊椎动物（草食动物）的相互作用，并借助条形码文库和eDNA数据集更详细地推断盐沼的食物网。条形码文库是必要的，因为盐沼中的许多植物表现出种内基因型变异或密切相关的物种及其杂交种共存。这种变化对动态生态系统物种组成的重要性是该提案的第二个焦点。