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Biosynthesis, transport and exudation of 1,4-benzoxazin-3-ones as determinants of plant biotic interactions

1,4-苯并恶嗪-3-酮的生物合成、运输和渗出作为植物生物相互作用的决定因素

基本信息

批准号：
243129298
负责人：
Professor Dr. Matthias Erb
金额：
--
依托单位：
Department of Animal and Plant Sciences
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/243129298?language=en
关键词：
Biosynthesis transport exudation benzoxazin ones

项目摘要

The establishment of a suitable biotic niche is essential for plant survival and agricultural productivity. One important mechanism by which plants shape their environment is the release of phytochemicals. Low molecular weight compounds in particular can initiate the interaction with beneficial microbes in the soil and ward off herbivores. However, the same signalling molecules may also be exploited by specialized pests and pathogens. A detailed understanding of their biosynthesis, transport and release will be essential to disentangle these seemingly contradicting effects and to harness the full potential of secondary metabolite exudation in sustainable cropping systems. Yet to date, no secondary metabolite exporters have been identified in crop model systems.Here we propose to unite the expertise of different research groups across Europe to study the molecular basis of 1,4-benzoxazin-3-one (BX) exudation in maize. Previous work by the consortium members has identified BXs as important resistance factors in maize and other grasses and has elucidated their biosynthesis in detail. We have also shown that BXs are the dominant secondary metabolites in maize root exudates and the leaf-apoplast and that they are important recruitment signals for beneficial microbes as well as for one of the most damaging pests of maize, the western corn rootworm Diabrotica virgifera. Given their obvious importance for crop productivity and their strong involvement in extracellular signalling, BXs are an ideal and relevant model to study the molecular ecology of secondary metabolite exudation. The overall aim of BENZEX is to create the most advanced molecular toolkit in extracellular plant-environment interactions to date. First, a comprehensive population of maize genotypes that are altered in BX biosynthesis will be established by interrupting or enhancing the expression of three important biosynthetic enzymes. Second targeted and untargeted reference methods to identify and quantify BX production, storage, exudation and transformation will be established using HPLC-MS and 13C-labelled BX precursors. Third, BX transporters will be identified by a combination of proteomics, quantitative PCR and phytochemically guided QTL-mapping. Fourth, the generated resources will be used to analyse the role of extracellular BXs in the interaction with the soil microbiome, plant growth promoting bacteria, arbuscular mycorrhizal fungi, root herbivores and leaf-feeding aphids. Taken together, our project will substantially increase knowledge about the biological relevance of secondary metabolite exudation. It will furthermore enhance the competitiveness of European molecular plant sciences by providing an array of new tools and resources, including transgenic plants and mutants, analytical methods as well as molecular and microbial markers for maize as an important agricultural model system.

建立一个合适的生物生态位对植物生存和农业生产力至关重要。植物塑造环境的一个重要机制是释放植物化学物质。特别是低分子量化合物可以引发与土壤中有益微生物的相互作用，并抵御食草动物。然而，同样的信号分子也可能被专门的害虫和病原体利用。详细了解它们的生物合成，运输和释放将是至关重要的解开这些看似矛盾的影响，并利用次生代谢物分泌的可持续种植系统的全部潜力。然而，迄今为止，没有次生代谢产物的出口商已被确定在作物模型systems. In这里，我们建议联合欧洲各地的不同研究小组的专业知识，研究1，4-benzoxazin-3-one（BX）在玉米渗出的分子基础。该联盟成员先前的工作已经确定BXs是玉米和其他禾本科植物中重要的抗性因子，并详细阐明了它们的生物合成。我们还表明，BXs是玉米根分泌物和叶质外体中占主导地位的次生代谢产物，并且它们是有益微生物以及玉米最具破坏性的害虫之一西方玉米根萤叶甲的重要招募信号。鉴于它们对作物生产力的明显重要性和它们在细胞外信号传导中的强烈参与，BX是研究次生代谢物分泌的分子生态学的理想和相关模型。BENZEX的总体目标是创建迄今为止最先进的细胞外植物-环境相互作用分子工具包。首先，将通过中断或增强三种重要生物合成酶的表达来建立BX生物合成改变的玉米基因型的综合群体。将使用HPLC-MS和13 C标记的BX前体建立第二种靶向和非靶向参考方法，以鉴定和定量BX的产生、储存、渗出和转化。第三，BX转运蛋白将通过蛋白质组学、定量PCR和植物化学引导的QTL定位的组合来鉴定。第四，产生的资源将用于分析细胞外BX在与土壤微生物组，植物生长促进细菌，丛枝菌根真菌，根食草动物和食叶蚜虫相互作用中的作用。总之，我们的项目将大大增加有关次级代谢产物分泌的生物相关性的知识。它还将通过提供一系列新的工具和资源，包括转基因植物和突变体、分析方法以及作为重要农业模式系统的玉米的分子和微生物标记，进一步提高欧洲分子植物科学的竞争力。