赤痢アメーバをモデルとした寄生虫ポリアミン生合成経路の新展開

完成了多胺生物合成途径中两种酶的纯化和性质研究。对溶组织内阿米巴多胺生物合成途径酶（鸟氨酸脱羧酶）进行了基因沉默和代谢组学分析。本项目中我们鉴定了一种寄生虫生存所必需的多胺途径酶，由于缺乏人类同源物，因此该酶成为一种新的药物靶点候选者。在本财政年度，在上一个财政年度，作为合著者出版了五份手稿。

Purification and complete characterization of two enzymes involved in polyamine biosynthesis pathway have been completed. Gene silencing and metabolome analysis of one polyamine biosynthesis pathway enzyme (Ornithine decarboxylase) in Entamoeba histolytica has been completed. In this project we have identified one polyamine pathway enzyme which is essential for parasite survival, and since human homologue was absent, therefore this enzyme emerged as a novel drug target candidateWe are going to submit two manuscript one related to glycerol biosynthesis and other related to hexosamine biosynthesis pathway in E. histolytica in this fiscal year. Five manuscripts as a coauthor have been published in the last fiscal year.