赤痢アメーバをモデルとした寄生虫ポリアミン生合成経路の新展開

多胺生物合成途径中涉及的两种酶的纯化和完全鉴定已经完成。溶组织内阿米巴中一种多胺生物合成途径酶(鸟氨酸脱羧酶)的基因沉默和代谢组分析已经完成。在本项目中，我们鉴定了一种对寄生虫的生存至关重要的多胺途径酶，由于缺乏人的同源物，因此该酶成为一种新的药物靶标候选。我们将在本财政年度提交两篇稿件，一篇与甘油生物合成有关，另一篇与己糖胺生物合成途径有关。上一财年出版了五篇合著者的手稿。

Purification and complete characterization of two enzymes involved in polyamine biosynthesis pathway have been completed. Gene silencing and metabolome analysis of one polyamine biosynthesis pathway enzyme (Ornithine decarboxylase) in Entamoeba histolytica has been completed. In this project we have identified one polyamine pathway enzyme which is essential for parasite survival, and since human homologue was absent, therefore this enzyme emerged as a novel drug target candidateWe are going to submit two manuscript one related to glycerol biosynthesis and other related to hexosamine biosynthesis pathway in E. histolytica in this fiscal year. Five manuscripts as a coauthor have been published in the last fiscal year.