赤痢アメーバをモデルとした寄生虫ポリアミン生合成経路の新展開

以溶组织内阿米巴为模型的寄生多胺生物合成途径的新进展

• 批准号: 21K15427
• 负责人: Jeelani Ghulam
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K15427/
• 关键词: Entamoeba histolytica; Protozoan parasite; Polyamine; Drug development; Metabolisms; Drug developement

Purification and complete characterization of two enzymes involved in polyamine biosynthesis pathway have been completed. Gene silencing and metabolome analysis of one polyamine biosynthesis pathway enzyme (Ornithine decarboxylase) in Entamoeba histolytica has been completed. In this project we have identified one polyamine pathway enzyme which is essential for parasite survival, and since human homologue was absent, therefore this enzyme emerged as a novel drug target candidateWe are going to submit two manuscript one related to glycerol biosynthesis and other related to hexosamine biosynthesis pathway in E. histolytica in this fiscal year. Five manuscripts as a coauthor have been published in the last fiscal year.

完成了多胺生物合成途径中两种酶的纯化和完整表征。完成了溶组织内阿米巴多胺生物合成途径酶（鸟氨酸脱羧酶）的基因沉默和代谢组学分析。在本项目中，我们发现了一种对寄生虫生存至关重要的多胺途径酶，由于缺乏人类同源物，因此该酶成为新的药物候选靶点。我们将在本财政年度提交两篇论文，一篇与甘油生物合成有关，另一篇与溶组织芽胞杆菌的己糖胺生物合成途径有关。在上一财政年度以共同作者的身份发表了五篇手稿。

项目成果

Isolation and Total Synthesis of Beru’amide, an Antitrypanosomal Polyketide from a Marine Cyanobacterium <i>Okeania</i> sp.

Beruamide 的分离和全合成，一种来自海洋蓝藻 <i>Okeania</i> sp 的抗锥虫聚酮化合物。

• DOI: 10.1021/acs.orglett.2c02013
• 发表时间: 2022
• 期刊: Organic Letters
• 影响因子: 5.2
• 作者: Taguchi Raimu;Iwasaki Arihiro;Ebihara Akira;Jeelani Ghulam;Nozaki Tomoyoshi;Suenaga Kiyotake
• 通讯作者: Suenaga Kiyotake

PTEN differentially regulates endocytosis, migration, and proliferation in the enteric protozoan parasite Entamoeba histolytica.

• DOI: 10.1371/journal.ppat.1010147
• 发表时间: 2022-05
• 期刊: PLoS pathogens
• 影响因子: 6.7

Transcriptome Analysis of Entamoeba histolytica Trophozoites during in vivo Contact-independent Mediated Host-parasite Interaction: Putative Pathways Related to PCD

• DOI: 10.9734/mrji/2022/v32i530390
• 发表时间: 2022-08
• 期刊: Microbiology Research Journal International
• 作者: D. G. P. Ishiwara;M. Shibayama;K. Nakada-Tsukui;G. Jeelani;María Del Consuelo Gómez-García;Olivia Medel Flores;T. Nozaki

Isolation and Total Synthesis of Kinenzoline, an Antitrypanosomal Linear Depsipeptide Isolated from a Marine Salileptolyngbya sp. Cyanobacterium

• DOI: 10.1021/acs.joc.1c00817
• 发表时间: 2021-08-31
• 期刊: JOURNAL OF ORGANIC CHEMISTRY
• 影响因子: 3.6
• 作者: Kurisawa, Naoaki;Otomo, Keisuke;Suenaga, Kiyotake
• 通讯作者: Suenaga, Kiyotake

Isolation and Total Synthesis of Bromoiesol sulfates, Antitrypanosomal arylethers from a Salileptolyngbya sp. Marine Cyanobacterium

• DOI: 10.1021/acs.joc.1c01214
• 发表时间: 2021-08-16
• 期刊: JOURNAL OF ORGANIC CHEMISTRY
• 影响因子: 3.6
• 作者: Ebihara, Akira;Iwasaki, Arihiro;Suenaga, Kiyotake
• 通讯作者: Suenaga, Kiyotake