Breast cancer tumors in deep tissue imaging with bi-modal NIR/MRI probe

在本财年，开发的双峰磁共振成像(MRI)和与赫赛汀(~40 nm)结合的近红外荧光探针用于靶向HER2乳腺癌。该探针由包裹IR-1061的PLGA-b-PEG胶束和包埋在PLGA/PEG界面上的Gd-四氮杂环十二烷四乙酸(Gd-DOTA)胶束组成。然后通过EDC/NHS化学方法将Herceptin通过它们的胺残基连接起来。本研究旨在评价所设计的用于乳腺癌检测的双峰成像探针的性能，并确定体外检测的最佳条件。为了评估该探针的检测限，我们评估了探针浓度的特异性结合活性。

In this fiscal year, the developed bimodal magnetic resonance imaging (MRI)/ and near-infrared fluorescence probe conjugating to Herceptin (~ 40 nm) for HER2 breast cancer targeting. The probe composed of PLGA-b-PEG micelles encapsulating IR-1061 in the core and gadolinium-tetraazacyclododecanetetraacetic acid (Gd-DOTA) conjugated at the border of PLGA/PEG. Herceptin was then conjugated through their amine residues via EDC/NHS chemistry. The study aims to assess the performance of the designed bimodal imaging probe for detecting of breast cancers and determine the optimal conditions in vitro in order to assess the detection limit of the probe, we evaluated the specific binding activities of probe concentrations.