An idea of regulatory science-based bone regeneration product for critical bone defect healing

用于关键骨缺损愈合的基于监管科学的骨再生产品的想法

• 批准号: 21K16962
• 负责人: Venkataiah VenkataSuresh
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K16962/
• 关键词: Regenerated Bone; Nanoindentation; Implant Therapy; Functional Bone; Micro-CT analysis; Calvaria osteoblasts; Polylactic acid scaffold; Critical bone defect; Bone tissue engineering; Bone product

The jawbones from the mice alveolar bone defect models were examined by Micro-CT scanning and histological analysis to assess new bone formation in the defect area at 4 and 8 weeks after implantation. The results of 3D image analysis and Hematoxylin and Eosin staining demonstrated that implanted MCOB-3DPL constructs have successfully regenerated bone within bone defects and is significantly higher than the other groups at 8 weeks post-implantation. Nanoindentation analysis has shown that regenerated bone induced by MCOB-3DPL constructs has micromechanical properties comparable to the native bone.The results of implant therapy by Micro-CT and histological analysis revealed that regenerated bone formed by MCOB-3DPL constructs possesses osseointegration ability.

种植后4周和8周，对小鼠牙槽骨缺损模型的颌骨进行Micro-CT扫描和组织学分析，以评估缺损区的新骨形成。3D图像分析和苏木精和伊红染色的结果表明，植入的MCOB-3DPL结构在骨缺损内成功再生骨，并且在植入后8周显著高于其他组。纳米压痕分析表明，MCOB-3DPL构建体诱导的再生骨具有与天然骨相当的微观力学性能，种植体治疗的Micro-CT和组织学分析结果表明，MCOB-3DPL构建体诱导的再生骨具有骨整合能力。

项目成果

Development of Bone Regeneration Product using Immature osteoblasts and three-dimensional polymer scaffold.

利用未成熟成骨细胞和三维聚合物支架开发骨再生产品。

• 发表时间: 2022
• 作者: Venkata Suresh Venkataiah;Shigeto Suzuki;Yoshio Yahata;Akira Kitagawa;Masahiko Inagaki;Mary M. Njuguna;Risako Nozawa;Yusuke Kakiuchi;Masato Nakano;Keisuke Handa;Masahiro Yamada;Hiroshi Egusa and Masahiro Saito
• 通讯作者: Hiroshi Egusa and Masahiro Saito

Development of bone tissue engineering technology using immature osteoblasts and three-dimensional polymer scaffold

利用未成熟成骨细胞和三维聚合物支架开发骨组织工程技术

• 发表时间: 2022
• 作者: Venkata Suresh Venkataiah;Shigeto Suzuki;Yoshio Yahata;Akira Kitagawa;Masahiko Inagaki;Mary M. Njuguna;Risako Nozawa;Yusuke Kakiuchi;Masato Nakano;Keisuke Handa;Masahiro Yamada;Hiroshi Egusa and Masahiro Saito
• 通讯作者: Hiroshi Egusa and Masahiro Saito