Cologne Experimental Eye Imaging Center (CEE-IC)

科隆实验眼部成像中心 (CEE-IC)

• 批准号: 405034447
• 负责人: Professor Dr. Felix Bock, since 7/2022
• 金额: --
• 依托单位: Institut für Biomedizinische Optik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/405034447?language=en
• 关键词: Cologne; Experimental; Eye; Imaging; Center

In the first funding period, a multimodal experimental imaging facility for mice, the Cologne Experimental Eye Imaging Center (CEE-IC), was established. In combination with specific acquisition and processing algorithms, which were developed for and adapted to the needs of the individual projects, the monitoring and classification of inflammatory ocular diseases have been made possible. In experimental studies using models for e.g. corneal inflammation, corneal edema and retinal degeneration, quantitative analyses including the three-dimensional quantification of pathological blood vessels in the same animals over time were carried out. In the planned second funding period, the facility will be extended with the aim of establishing specific optical coherence tomography (OCT) imaging on a cellular level. For this purpose, a microscopic OCT (mOCT) system will be installed, which allows for non-invasive in vivo investigation of murine eyes in three dimensions with a resolution down to 1 micrometer. Furthermore, the use of specifically addressed nanoparticles for quantification of ocular surface inflammation will be explored. The potential for the clinical translation of the innovative hardware and software approaches will be evaluated. Altogether, the new possibilities will bring advantages for all involved projects. For example, non-invasive imaging of clinically invisible lymphatic vessels including the visualization of intraluminal cells will be possible in the same animals over time. Using mOCT will make structural and angiographic OCT imaging more competitive to invasive histology and consequently has the potential to significantly reduce the number of animals in preclinical research.

在第一个资助期内，建立了一个用于小鼠的多模式实验成像设施，即科隆实验眼成像中心（CEE-IC）。结合针对各个项目需求开发的特定采集和处理算法，炎症性眼病的监测和分类成为可能。在使用例如角膜炎症、角膜水肿和视网膜变性的模型的实验研究中，进行定量分析，包括随着时间的推移在相同动物中病理血管的三维定量。在计划的第二个供资期内，该设施将得到扩展，目的是在细胞水平上建立特定的光学相干断层扫描成像。为此，将安装显微光学相干断层扫描（mOCT）系统，该系统允许在分辨率低至1微米的三维空间内对小鼠眼睛进行无创体内研究。此外，将探索使用特定寻址的纳米颗粒来定量眼表炎症。将评估创新硬件和软件方法的临床转化潜力。总之，新的可能性将为所有相关项目带来优势。例如，随着时间的推移，在相同的动物中，临床上不可见的淋巴管的非侵入性成像（包括管腔内细胞的可视化）将是可能的。使用mOCT将使结构和血管造影OCT成像比侵入性组织学更具竞争力，因此有可能显著减少临床前研究中的动物数量。