Spatiotemporal metabolomics analysis in the human cornea-on-a-chip for the determination of ocular drug toxicity

人角膜芯片时空代谢组学分析用于测定眼部药物毒性

• 批准号: 22K14548
• 负责人: ABDALKADER Rodi
• 金额: $ 2.91万
• 依托单位: Ritsumeikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K14548/
• 关键词: Cornea-on-a-chip; Microfluidic; Metabolomic; Drug toxicity; cornea-on-a-chip; iPSC; metabolomics

This research aims to identify metabolite markers related to drug toxicity using untargeted metabolomic analysis in a human corneal epithelium-on-a-chip. In the current fiscal year, an untargeted metabolic workflow that we previously developed was utilized to analyze extracellular metabolites in human induced pluripotent stem cells (hiPSCs) under early differentiation conditions. A total of 117 metabolites were successfully annotated in a small sample volume of one microliter. This optimized untargeted metabolomic method will be applied to the human corneal epithelium-on-a-chip in the next phase of the study.

本研究的目的是在人角膜上皮芯片中使用非靶向代谢组学分析来鉴定与药物毒性相关的代谢物标志物。在本财政年度，我们之前开发的非靶向代谢工作流程被用于分析早期分化条件下人类诱导多能干细胞（hiPSC）中的细胞外代谢物。在1微升的小样品体积中成功注释了总共117种代谢物。这种优化的非靶向代谢组学方法将在下一阶段的研究中应用于人角膜上皮芯片。

项目成果

The survival and characteristics of cryopreserved human iPSC-derived corneal epithelial cells generated by SSM-CI method

SSM-CI 方法产生的冻存人 iPSC 来源的角膜上皮细胞的存活和特性

• 发表时间: 2022
• 作者: Abdalkader Rodi;Chaleckis Romanas;Fujita Takuya;Rodi ABDALKADER
• 通讯作者: Rodi ABDALKADER

Personal website

个人网站

Early Differentiation Signatures in Human Induced Pluripotent Stem Cells Determined by Non-Targeted Metabolomics Analysis

通过非靶向代谢组学分析确定人类诱导多能干细胞的早期分化特征

• DOI: 10.1101/2023.03.02.530741
• 发表时间: 2023
• 期刊: bioRxiv
• 作者: Abdalkader Rodi;Chaleckis Romanas;Fujita Takuya
• 通讯作者: Fujita Takuya

ヒト角膜・オン・チップの開発

人类角膜芯片的开发

• 发表时间: 2022
• 作者: Abdalkader Rodi;Chaleckis Romanas;Fujita Takuya;Rodi ABDALKADER;Rodi ABDALKADER
• 通讯作者: Rodi ABDALKADER