Spatiotemporal metabolomics analysis in the human cornea-on-a-chip for the determination of ocular drug toxicity

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This research aims to identify metabolite markers related to drug toxicity using untargeted metabolomic analysis in a human corneal epithelium-on-a-chip. In the current fiscal year, an untargeted metabolic workflow that we previously developed was utilized to analyze extracellular metabolites in human induced pluripotent stem cells (hiPSCs) under early differentiation conditions. A total of 117 metabolites were successfully annotated in a small sample volume of one microliter. This optimized untargeted metabolomic method will be applied to the human corneal epithelium-on-a-chip in the next phase of the study.