Autonomous genome replication and protein synthesis in a cell-free system under microfluidic accumulation and feeding (P08)

微流控积累和补给下无细胞系统中的自主基因组复制和蛋白质合成（P08）

• 批准号: 405567376
• 金额: --
• 依托单位: Lehrstuhl für Experimentelle Physik - Biophysik
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/405567376?language=en
• 关键词: Autonomous; genome; replication; protein; synthesis

We will study plausible geological non-equilibrium environments composed of open rock pores for their suitability to continuously supply biochemical reaction networks driving replication and expression of genetic information. The aim is to host and position these complex reaction networks over an extended period of time through physical disequilibria without the need for membranes, while maintaining their activity. We will use recombinant in vitro translation systems coupled to DNA replication and energy regeneration as a proxy for a metabolic system capable of maintaining both flow of genetic information as well as a minimal metabolism.

我们将研究由开放的岩石孔隙组成的合理的地质非平衡环境，以确定其是否适合持续提供生物化学反应网络，从而驱动遗传信息的复制和表达。其目的是通过物理不平衡在较长时间内托管和定位这些复杂的反应网络，而无需膜，同时保持其活性。我们将使用与DNA复制和能量再生偶联的重组体外翻译系统作为能够维持遗传信息流和最小代谢的代谢系统的代表。