Development of novel Immunosynbiotics with wakame utilizing immunobiotics and immunogenics

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Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. This year, three results were achieved in this study:(1)Eight lactobacilli strains selected in previous studies were able to grow better in wakame-based broths with the addition of B vitamins. The comparative genomic analysis revealed that the differences between the strains were related to their different wakame utilizing abilities.(2)One strain that had adhesion ability to porcine intestinal epithelial cells could survive under the simulated gastric juice in the presence of wakame because the fiber in wakame could protect the cells from the severe environment of acid.(3)The growth and storage conditions have various effects on the characteristics of lactic acid bacteria, especially high-temperature and low-temperature stress The incubation condition of the selected strain was determined. In future research, the strain under determined incubation conditions will be provided for the in vivo study.