Development of novel Immunosynbiotics with wakame utilizing immunobiotics and immunogenics

利用免疫生学和免疫原性开发裙带菜新型免疫合生素

• 批准号: 22K14982
• 负责人: Binghui Zhou
• 金额: $ 3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K14982/
• 关键词: Immunobiotics; Immunosynbiotics; Wakame; Lactobacilli; Porcine intestinal cell; Tolerance to acid; Porcine; Probiotics; Immunogenics

Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. This year, three results were achieved in this study:(1)Eight lactobacilli strains selected in previous studies were able to grow better in wakame-based broths with the addition of B vitamins. The comparative genomic analysis revealed that the differences between the strains were related to their different wakame utilizing abilities.(2)One strain that had adhesion ability to porcine intestinal epithelial cells could survive under the simulated gastric juice in the presence of wakame because the fiber in wakame could protect the cells from the severe environment of acid.(3)The growth and storage conditions have various effects on the characteristics of lactic acid bacteria, especially high-temperature and low-temperature stress The incubation condition of the selected strain was determined. In future research, the strain under determined incubation conditions will be provided for the in vivo study.

在畜牧业生产中过度使用抗生素导致的抗生素耐药性一直是一个严重的问题，导致需要有效的替代品。抗生素的一个有前途的替代品是“免疫合生元”，一种免疫生物素和免疫益生元的组合。海藻裙带菜被认为是一种免疫益生菌，L。salivarius是一种从喂饲wakame的猪的肠中分离的免疫生物素。在猪饲料中添加裙带菜已被发现可以改善免疫反应并改变猪大肠中的微生物群。使用裙带菜作为饲料补充剂也可以为2011年东北地震后的宫城县提供经济复苏的替代方案。本年度的研究取得了三项成果：（1）在以往的研究中筛选出的8株乳酸杆菌菌株，在添加了B族维生素的裙带菜肉汤中能够更好地生长。比较基因组分析表明，不同菌株间的差异与它们对裙带菜的利用能力不同有关。（2）一株对猪小肠上皮细胞具有粘附能力的菌株能在裙带菜存在的模拟胃液中存活，这是因为裙带菜中的纤维可以保护细胞免受酸性环境的影响。（3）生长和贮藏条件对乳酸菌的特性有不同程度的影响，尤其是高温和低温胁迫对乳酸菌的特性影响较大。在未来的研究中，将提供在确定的孵育条件下的菌株用于体内研究。

项目成果

Basic research on growth improvement of immunosynbiotic

免疫合生素促进生长的基础研究

• 发表时间: 2022
• 作者: 周 冰卉;劉 瑛琦;櫻井 美月;AKM Humayun Kober;Muhammad Shahid Riaz Rajoka;大坪 和香子;Julio Villena;北澤 春樹
• 通讯作者: 北澤 春樹

Survival of immunobiotics in the GI tract for immunosynbiotic development

免疫抗生素在胃肠道中的存活以促进免疫合生素的发展

• 发表时间: 2022
• 作者: Binghui Zhou;AKM Humayun Kober;Muhammad Shahid Riaz Rajoka;Wakako Ikeda-Ohtsubo;Julio Villena;Haruki Kitazawa
• 通讯作者: Haruki Kitazawa