Study of enzymes inside liquid-liquid phase separated crowded droplets

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We recently discovered for the first time that enzymatically active artificial protocells are able to move in solution following a chemical gradient. The pH change generated by the enzyme within the droplets drives the droplets movement in chemotactic manner. We have investigated on the molecular mechanism in detail by means of biochemical, imaging and fluid mechanism studies. We also demonstrated that these droplets are able to reconstitute a simple and linear protometabolic pathway. We are currently studying the catalytic efficiency of several enzymes inside our liquid-liquid phase separated protocells.