Study on brassinosteroid-mediated regulation of stem cell regeneration in roots

油菜素类固醇介导的根干细胞再生调控研究

• 批准号: 22K15143
• 负责人: ZHANG YE
• 金额: $ 3万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15143/
• 关键词: stem cell; DNA damage; cell division; regeneration; brassinosteroid; plant stem cell; DNA damage response

I have experimentally tested the effects of brassinosteriod (brassinolide) application and the effects of inhibiting brassinosteroid biosynthesis (by brassinozole treatment) on DNA damage induced stem cell death and the replenishing cell division afterwards. The results showed that brassinosteroid plays a positive role both in promoting DNA damage-induced stem cell death and the replenishing cell division afterwards.I have examined the regeneration capacity of transgenic plants in which the feedback activation on BRL3 expression is defective. Quantification analysis revealed that the feedback defective lines showed less replenishing cell division than brl3 mutant but not comparable to the wildtype, indicating that the feedback effect BRL3 contributes to activate replenishing cell division.Using translational fusion of fluorescent proteins, I have examined the expression pattern of BRL3 in the root tip during genotoxic stress exposure (before and after stem cell death) and during the afterward stem cell regeneration phase. The result revealed a positive correlation between the expression level of BRL3 and the activation of replenishing cell division. At the time point prior to the activation of replenishing cell division, the expression level of the mutant version of BRL3, on which the feedback activation is defective, was significantly lower than that of the wild type BRL3.

我已经通过实验测试了Brassinosteriod（Brassinolide）施用的影响以及抑制铜氨基固醇生物合成的影响（通过脑诺唑治疗）对DNA损伤诱导的干细胞死亡和随后补充细胞分裂的影响。结果表明，腕素类固醇在促进DNA损伤诱导的干细胞死亡和后来补充细胞分裂方面起着积极作用。I已检查了转基因植物的再生能力，其中BRL3表达的反馈激活是有缺陷的。量化分析表明，反馈有缺陷的线比BRL3突变体的补充细胞分裂较少，但与野生型不相当，这表明反馈效应BRL3有助于激活荧光蛋白的翻译融合。在基因毒素plosection Plosecomection septrosiation and the Pellsen pline pline selter中，我已经检查了BRL3的平移融合，并在Pelternef inter tement selter pline theard the the efternard Cellece searder grounder（和之后）。结果表明，BRL3的表达水平与补充细胞分裂的激活之间存在正相关。在补充细胞分裂激活之前的时间点，反馈激活有缺陷的突变版本的表达水平明显低于野生型BRL3的表达水平。