Molecular analysis of novel wound-induced regulators of shoot regeneration in Arabidopsis

拟南芥中新型伤口诱导芽再生调节因子的分子分析

基本信息

  • 批准号:
    22K15150
  • 负责人:
  • 金额:
    $ 3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
  • 财政年份:
    2022
  • 资助国家:
    日本
  • 起止时间:
    2022-04-01 至 2023-03-31
  • 项目状态:
    已结题

项目摘要

In FY 2022 My main contribution to this project is characterizing how the wound-activated HEAT SHOCK FACTOR A1 (HSFA1) promotes shoot regeneration in Arabidopsis. I utilized a combination of genetic, biochemical and bioinformatic tools to reveal a novel mechanism of wounding-induced shoot regeneration.Key research findings: 1) Determined key downstream targets of HSFA1 using a bioinformatics approach. 2) Verified HSFA1 binding to direct targets using biochemistry. 3) Generated and phenotyped genetic crosses to confirm epistasis of genes involved in wound-induced cellular reprograming. 4) Observed the nuclear translocation of HSFA1d in response to wounding using live -The results have been compiled into a manuscript for submission in 2023Collaborations (informal)1) Ari Sadanandom (Durham University, UK) – Shared plant material and jointly wrote a grant proposal to Biotechnology and Biological Sciences Research Council (BBSRC) to investigate SUMOylation in cellular reprograming. 2) Teruaki Taji (Tokyo University of Agriculture) – Provided cDNA sample for qPCR analysis to investigate the role of HSFA1 in photomorphogenesis. 3) Yutaka Kodama (Utsunomiya University) – Visited laboratory and learned techniques for imaging leave epidermal cells with a confocal microscope.Conferences: 1)Oral - Annual conference for Society of Experimental Biology, Montpellier France.2) Oral - Satellite meeting on SUMO modification in cell signalling – Society of Experimental Biology, Montpellier France – Invited speaker. 3)Poster - CSRS Annual progress meeting 2022
在2022财年,我对该项目的主要贡献是表征伤口激活的热休克因子A1(HSFA 1)如何促进拟南芥的芽再生。本研究综合运用遗传学、生物化学和生物信息学等多种手段,揭示了创伤诱导再生的新机制。主要研究成果:1)利用生物信息学方法确定了HSFA 1的下游关键靶基因。2)使用生物化学验证HSFA 1与直接靶标的结合。3)产生和表型遗传杂交,以确认参与创伤诱导细胞重编程的基因上位性。4)观察到HSFA 1d的核易位,以响应使用活的创伤-结果已汇编成手稿提交2023年合作(非正式)1)阿里Sadanandom(达勒姆大学,英国)共享植物材料,并共同撰写了一份拨款建议,生物技术和生物科学研究理事会(BBSRC),以调查细胞重编程中的SUMO化。2)Teruaki Taji(Tokyo University of Agriculture)提供cDNA样品用于qPCR分析,以研究HSFA 1在光形态建成中的作用。3)儿玉丰(宇都宫大学)参观了实验室,学习了用共聚焦显微镜对叶表皮细胞进行成像的技术。会议:1)Oral -法国蒙彼利埃实验生物学学会年会。2)Oral -法国蒙彼利埃实验生物学学会SUMO修饰细胞信号的卫星会议特邀演讲人。3)海报- CSRS 2022年度进展会议

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The role of SUMOylation during shoot regeneration in Arabidopsis
SUMO化在拟南芥芽再生过程中的作用
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Morinaka Hatsune;Coleman Duncan;Sugimoto Keiko;Iwase Akira;Duncan Coleman
  • 通讯作者:
    Duncan Coleman
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Coleman Duncan其他文献

Coleman Duncan的其他文献

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