Molecular analysis of novel wound-induced regulators of shoot regeneration in Arabidopsis

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In FY 2022 My main contribution to this project is characterizing how the wound-activated HEAT SHOCK FACTOR A1 (HSFA1) promotes shoot regeneration in Arabidopsis. I utilized a combination of genetic, biochemical and bioinformatic tools to reveal a novel mechanism of wounding-induced shoot regeneration.Key research findings: 1) Determined key downstream targets of HSFA1 using a bioinformatics approach. 2) Verified HSFA1 binding to direct targets using biochemistry. 3) Generated and phenotyped genetic crosses to confirm epistasis of genes involved in wound-induced cellular reprograming. 4) Observed the nuclear translocation of HSFA1d in response to wounding using live -The results have been compiled into a manuscript for submission in 2023Collaborations (informal)1) Ari Sadanandom (Durham University, UK) – Shared plant material and jointly wrote a grant proposal to Biotechnology and Biological Sciences Research Council (BBSRC) to investigate SUMOylation in cellular reprograming. 2) Teruaki Taji (Tokyo University of Agriculture) – Provided cDNA sample for qPCR analysis to investigate the role of HSFA1 in photomorphogenesis. 3) Yutaka Kodama (Utsunomiya University) – Visited laboratory and learned techniques for imaging leave epidermal cells with a confocal microscope.Conferences: 1)Oral - Annual conference for Society of Experimental Biology, Montpellier France.2) Oral - Satellite meeting on SUMO modification in cell signalling – Society of Experimental Biology, Montpellier France – Invited speaker. 3)Poster - CSRS Annual progress meeting 2022