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国内に現存する氷河を拠点に据えた氷河生態系の時空間的多様性解析

基于日本现有冰川的冰川生态系统时空多样性分析

基本信息

批准号：
22K15187
负责人：
村上匠
金额：
$ 2.91万
依托单位：
National Institute of Genetics
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2026-03-31
项目状态：
未结题

项目摘要

氷河表面の雪氷上には様々な微生物や小動物が生息している。こうした氷河生物の活動が流域の物質循環や氷河の融解と密接に関連していることが明らかとなってきており、気候変動が氷河生態系の構造にどのような影響を与えるのか注目が集まっている。一方で、氷河微生物の多様性や生態に関しては未だ不明な点が多く残されている。特に、極域以外の地域の氷河生態系に関しては情報が不足している。本課題では、国内に現存する氷河に着目し、表面に生息する微生物、特に細菌群集のメタゲノムを継続的に収集・解析することを目標としている。そうした集中的かつ大規模な解析を行うことで、氷河細菌群集の系統組成や代謝能の詳細を把握し、従来調査の及んで来なかった日本の氷河生態系の詳細解明を目指す。さらに、これまで公開された海外氷河メタゲノムデータと統合した比較解析を行うことによって。日本を含めた世界各地の氷河細菌群集構造の相違点を詳らかにし、氷河生態系の地球規模での多様性を明らかにする予定である。2022年度は、富山県立山の氷河にて8月と10月の2回の雪氷試料採集を実施した。現在、これら試料について核酸試料を調製し、配列解析に向けて準備している。一方、世界各地の氷河メタゲノムデータ解析については、2022年度に海外のチームから報告されたデータセットを加えた統合解析を実施した。その結果、極域やアジア内陸部など様々な地域の氷河細菌群集について、系統組成や保有する遺伝子、メタゲノム配列から再構築された各細菌種のドラフトゲノム情報など、今後比較解析を進めていく上で基盤となるデータを作成した。

Microorganisms and small animals live on the snow on the surface of the river. The biological activities of the river basin are related to the material circulation of the river basin, the melting and close connection of the river basin, and the structure of the river ecosystem is affected by the changes of weather and climate. The diversity of microorganisms in the river and the river is closely related to ecology. There is insufficient information about the Pohe ecosystem in areas outside the polar region. The subject is to analyze the collection and distribution of microorganisms and bacteria living on the surface of existing rivers in China. A large-scale analysis of the concentration of bacteria, a detailed understanding of metabolic energy, a recent survey, and a detailed understanding of the ecosystem of the river in Japan were conducted. This is the first time that we've had a chance to talk to each other. The diversity of the Earth's size of the river ecosystem in Japan includes detailed structural differences among the river bacterial communities around the world In 2022, the collection of snow samples was carried out in August and October of the year. Now, sample preparation, nucleic acid sample preparation, alignment analysis and preparation. In 2022, we will conduct comprehensive analysis of overseas projects. The results show that the population of bacteria in the river is divided into three parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into four parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into four parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into four parts: the population of bacteria in the inland part of the river is divided into three parts: the population of bacteria in the inland part of the river is divided into four parts: the population of bacteria in the inland part of the river is divided into four parts: the inland part of bacteria in the inland part of the inland part of the river is divided into four parts: the population is divided into four parts: the population of bacteria in the inland part of the inland part of the river is