Elucidating the circuits of functional lateralization in the hippocampus

阐明海马体功能偏侧化的回路

• 批准号: 22K15234
• 负责人: Guan Hefei
• 金额: $ 2.83万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15234/
• 关键词: Spatial memory; Hippocampus; Lateralization; Memory mechanisms

The first stage of the project involved characterizing the inhibitory effects and time course of viral expression of Gi-DREADD and KOR-DREADD in the hippocampus, as well as examining their corresponding ligand effects on in vivo hippocampal activity.For the second stage, behavioral tasks were conducted to investigate the different functions of the left and right CA3 regions in spatial navigation and memory. In the object location test, mice were trained with two familiar objects, and during the second session of recall, one of the objects was moved to a different location after the left CA3 was silenced instead of the right CA3. The mice spent more time exploring the object in the novel location, which is attributed to their innate preference for novelty. These findings suggest that the left CA3 region is more crucial for the acquisition of spatial memory.

第一阶段研究Gi-DREADD和KOR-DREADD对海马病毒表达的抑制作用和时间过程，以及相应的配体对海马活性的影响;第二阶段通过行为任务研究左右CA 3区在空间导航和记忆中的不同功能。在物体定位测试中，小鼠接受了两个熟悉物体的训练，在第二次回忆期间，在左侧CA 3而不是右侧CA 3沉默后，其中一个物体被移动到不同的位置。老鼠花更多的时间探索新位置的物体，这归因于它们对新奇事物的先天偏好。这些发现表明，左CA 3区是更重要的空间记忆的收购。

项目成果

The expanded circuitry of hippocampal ripples and replay

海马波纹和重放的扩展电路

• DOI: 10.1016/j.neures.2022.12.010
• 发表时间: 2023
• 期刊: Neuroscience Research
• 影响因子: 2.9
• 作者: He Hongshen;Guan Hefei;McHugh Thomas J.
• 通讯作者: McHugh Thomas J.