Planctomycetes as a source of novel secondary metabolites: triggers and regulation of their biosynthesis and engineered production in native strains or heterologous hosts

浮霉菌作为新型次生代谢物的来源：其生物合成和工程生产在天然菌株或异源宿主中的触发和调节

• 批准号: 405562673
• 负责人: Dr. Nicolai Kallscheuer
• 金额: --
• 依托单位: Professur für Mikrobielle Interaktionen
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/405562673?language=en
• 关键词: Planctomycetes; source; novel; secondary; metabolites

Planctomycetes represent a phylum of environmentally important bacteria often associated with phototrophs such as macro- and microalgae and thus play a key role in global carbon and nitrogen cycles. Several planctomycetal species grow slowly with doubling times of up to one month. Most other heterotrophs occupying same ecological niches divide much faster but fail to outcompete Planctomycetes. It is assumed that the high abundance of Planctomycetes in marine environments results from the synthesis of secondary metabolites, which might act as potent antimicrobials or algicidals. This notion is further supported by the complex life cycle of Planctomycetes and quite large genomes for prokaryotes, characteristics that are typical of bacteria known for their bioactive potential. The proposed project aims at a detailed characterization of the secondary metabolome of planctomycetal species. Beyond the identification of novel planctomycetal secondary metabolites, in particular the underlying biosynthetic pathways and the regulation of their production (e.g. as response to environmental triggers) will be analyzed in more detail. Biosynthetic pathways for interesting secondary metabolites will subsequently be reconstructed by functional expression of planctomycetal genes in genetically manipulable microbial host organisms (including already genetically accessible Planctomycetes). This strategy can provide access to individual compounds in amounts required for a detailed analysis of presumed effects.

植物菌是一类对环境具有重要意义的细菌，通常与巨藻和微藻等光养生物有关，因此在全球碳和氮循环中起着关键作用。一些植物种类生长缓慢，可达一个月翻倍。大多数占据相同生态位的其他异养生物分裂得更快，但无法与植物菌竞争。据推测，海洋环境中浮游菌的高丰度是次生代谢物合成的结果，次生代谢物可能作为有效的抗菌剂或杀藻剂。这一观点得到了植物菌复杂的生命周期和原核生物相当大的基因组的进一步支持，这些特征是已知具有生物活性潜力的细菌的典型特征。该项目旨在详细表征植物植物的次生代谢组。除了鉴定新的植物次生代谢物之外，特别是潜在的生物合成途径及其产生的调节（例如对环境触发的反应）将进行更详细的分析。有趣的次生代谢物的生物合成途径随后将通过在遗传上可操纵的微生物宿主生物（包括已经遗传上可接近的plantomycetes）中plantomycetal基因的功能表达来重建。这一策略可以提供对假定影响进行详细分析所需数量的个别化合物。