Multiple functions of Adipsin in adipocyte-breast cancer cell interaction

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We isolated adipose-derived stem cells (ADSC) from the mammary fat pad of wild-type (WT) and Adipsin knockout (KO) mice and differentiated them into mature adipocytes.1. Adipsin-KO inhibits the secretion of several cytokines and adipokines, including macrophage chemoattractant protein-1 (MCP-1) as determined by cytokine array analysis of cell culture medium from mature adipocytes.2. Using a syngeneic mouse model of breast cancer, we discovered that the mammary fat pad tumors of Adipsin-KO mice are smaller than those of wild-type mice.RT-qPCR analysis of mature Adipsin-KO and WT adipocytes revealed differential gene expression of multiple genes involved in fat droplet formation and biosynthesis.3. Using a high-fat diet, we found that Adipsin-KO mice are resistant to diet-induced obesity.