Multiple functions of Adipsin in adipocyte-breast cancer cell interaction

Adipsin 在脂肪细胞-乳腺癌细胞相互作用中的多种功能

• 批准号: 22K15532
• 负责人: Behnoush Khaledian
• 金额: $ 2.91万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15532/
• 关键词: Adipsin; Adipocyte; Breast cancer; Cancer stem cell

We isolated adipose-derived stem cells (ADSC) from the mammary fat pad of wild-type (WT) and Adipsin knockout (KO) mice and differentiated them into mature adipocytes.1. Adipsin-KO inhibits the secretion of several cytokines and adipokines, including macrophage chemoattractant protein-1 (MCP-1) as determined by cytokine array analysis of cell culture medium from mature adipocytes.2. Using a syngeneic mouse model of breast cancer, we discovered that the mammary fat pad tumors of Adipsin-KO mice are smaller than those of wild-type mice.RT-qPCR analysis of mature Adipsin-KO and WT adipocytes revealed differential gene expression of multiple genes involved in fat droplet formation and biosynthesis.3. Using a high-fat diet, we found that Adipsin-KO mice are resistant to diet-induced obesity.

我们从野生型（WT）和Adipsin敲除（KO）小鼠的乳腺脂肪垫中分离脂肪源性干细胞（ADSC）并将其分化为成熟脂肪细胞。Adipsin-KO抑制几种细胞因子和脂肪因子的分泌，包括巨噬细胞趋化蛋白-1（MCP-1），如通过来自成熟脂肪细胞的细胞培养基的细胞因子阵列分析所确定的。利用同基因小鼠乳腺癌模型，我们发现Adipsin-KO小鼠乳腺脂肪垫肿瘤比野生型小鼠小，成熟Adipsin-KO和WT脂肪细胞的RT-qPCR分析显示了参与脂肪滴形成和生物合成的多个基因的差异表达.使用高脂肪饮食，我们发现Adipsin-KO小鼠对饮食诱导的肥胖有抵抗力。