Multiple functions of Adipsin in adipocyte-breast cancer cell interaction

我们从野生型(WT)和脂肪酶基因敲除(KO)小鼠的乳房脂肪垫中分离出脂肪干细胞(ADSC)，并将其分化为成熟脂肪细胞。通过对成熟脂肪细胞培养上清液的细胞因子阵列分析，发现Adipsin-KO抑制多种细胞因子和脂肪因子的分泌，包括巨噬细胞趋化蛋白-1(MCP-1)。利用同基因小鼠乳腺癌模型，我们发现Adipsin-KO小鼠的乳腺脂肪垫肿瘤比野生型小鼠小。成熟的Adipsin-KO和WT脂肪细胞的RT-qPCR分析显示，参与脂肪滴形成和生物合成的多个基因存在差异表达。使用高脂肪饮食，我们发现Adipsin-KO小鼠对饮食诱导的肥胖具有抵抗力。

We isolated adipose-derived stem cells (ADSC) from the mammary fat pad of wild-type (WT) and Adipsin knockout (KO) mice and differentiated them into mature adipocytes.1. Adipsin-KO inhibits the secretion of several cytokines and adipokines, including macrophage chemoattractant protein-1 (MCP-1) as determined by cytokine array analysis of cell culture medium from mature adipocytes.2. Using a syngeneic mouse model of breast cancer, we discovered that the mammary fat pad tumors of Adipsin-KO mice are smaller than those of wild-type mice.RT-qPCR analysis of mature Adipsin-KO and WT adipocytes revealed differential gene expression of multiple genes involved in fat droplet formation and biosynthesis.3. Using a high-fat diet, we found that Adipsin-KO mice are resistant to diet-induced obesity.