Infection free and scalable production of DNA oligonucleotides for mass production of nano-objects by self-assembling

在将dna分子广泛应用于纳米技术的道路上，对生物体的研究是下一个里程碑。对于后者以及DNA纳米物体在材料科学中的应用研究，单链DNA （ss-DNA）的可扩展供应将是必要的。因此，需要新的ss-DNA生产工艺来实现从克到公斤甚至更大的进步。为了有效地利用生物技术大规模生产已确定的ss-DNA对象，必须利用现有的生物技术生产工厂。合同制造商不可能使用感染性噬菌体M13生产ss-DNA支架，或使用辅助噬菌体与噬菌体结合生产用户定义的和人工的ss-DNA，因为在大肠杆菌进行的所有其他生产过程中，都存在不必要的噬菌体感染风险。因此，本项目的目的是合理设计一种新的工艺，用于在单锅反应中自组装纳米物体的无感染和高效生产用户定义的ss-DNA。为此，在大肠杆菌中使用的不是传染性的辅助噬菌体，而是与噬菌体结合的辅助质粒。

Studies in living organisms are the next milestone on the way to broad applicability of DNA-molecules for nanotechnology. For the latter as well as for studies on the application of DNA nano-objects in material sciences, the scalable supply of single-stranded-DNA (ss-DNA) will be necessary on a kilogram scale. Thus new ss-DNA production processes are required to make the step forward from gram to kilogram scale and even further. To enable the efficient biotechnological mass production of defined ss-DNA objects, it is essential to make use of existing biotechnological production plants. Using the infectious bacteriophage M13 for ss-DNA scaffold production or using a helperphage in combination with a phagemid for the production of user-defined and artificial ss-DNA with Escherichia coli is impossible with contract manufacturers, as the risk of unwanted phage infections persists throughout all other production processes performed there with E. coli. Thus the aim of this project is the rational design of a new process for infection free and efficient production of user-defined ss-DNA for self-assembly of nano-objects in a single-pot reaction. For this, instead of an infectious helperphage, a helperplasmid in combination with a phagemid is supposed to be used in E. coli.