Scanning dynamics of the mammalian ribosome during translation initiation (A15*)

翻译起始过程中哺乳动物核糖体的扫描动力学 (A15*)

• 批准号: 405772023
• 金额: --
• 依托单位: Institut für Mikrobiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/405772023?language=en
• 关键词: Scanning; dynamics; mammalian; ribosome; during

Translation of most eukaryotic mRNAs starts by a scanning mechanism. The small ribosomal subunit is recruited to the mRNA 5’ end and then migrates in 3’ direction towards the AUG start codon. Cells have developed multiple mechanisms to regulate the scanning process and thus shape their proteome. Misregulation can lead to diseases such as cancer and viral infections. We will study scanning using single molecule Förster Resonance Energy Transfer (smFRET) and Total Internal Reflection of Fluorescence (TIRF) microscopy. Our work will establish the mechanisms of function and regulation of gene expression during translation in eukaryotes.

大多数真核生物mRNA的翻译都是从扫描机制开始的。核糖体小亚基被募集到mRNA 5'端，然后以3'方向向AUG起始密码子迁移。细胞已经开发出多种机制来调节扫描过程，从而塑造它们的蛋白质组。调节不当会导致癌症和病毒感染等疾病。我们将研究使用单分子Förster共振能量转移（smFRET）和荧光全内反射（TIRF）显微镜扫描。我们的工作将建立真核生物翻译过程中基因表达的功能和调控机制。