Scanning dynamics of the mammalian ribosome during translation initiation (A15*)

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Translation of most eukaryotic mRNAs starts by a scanning mechanism. The small ribosomal subunit is recruited to the mRNA 5’ end and then migrates in 3’ direction towards the AUG start codon. Cells have developed multiple mechanisms to regulate the scanning process and thus shape their proteome. Misregulation can lead to diseases such as cancer and viral infections. We will study scanning using single molecule Förster Resonance Energy Transfer (smFRET) and Total Internal Reflection of Fluorescence (TIRF) microscopy. Our work will establish the mechanisms of function and regulation of gene expression during translation in eukaryotes.