Establishment of a novel platform of Raman microscope for diagnosis of hydrogel-generated cancer stem cells.

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During this fiscal year was investigated the action of PAMPS gel on myoblast cell models. It was found that Poly (2-acrylamido-2- methyl-1-propanesulphonic acid) hydrogel have a significant impact to induce an elevation of stemness markers such as Sox2, Nanog, and Oct3/4. This elevation of stemness markers have been linked to a change of cell morphology, meaning that cell that have a higher expression level tend to be more round and small whereas the one that have a low expression of stemness markers tend to be bigger and flat. This interesting fact showed that by stainingThe first Raman images of the mentioned hydrogel-cell interaction system was performed with a high-speed slit-scanning Raman microscope. The quality of the measurement was performed with different metric such as signal-to-noise ratio, Laplacian operator and non-homogenous line illumination quantification. As a result the measurement protocol for this complex measurement provide high-quality data.To exploit Hyperspectral Raman images on hydrogel substrate, two algorithms that allow to compare Data from different Raman devices and different dates of measurement was finalized. One algorithm is for single cell measurement. Another one is for 3D colony formation measurement.