Establishment of a novel platform of Raman microscope for diagnosis of hydrogel-generated cancer stem cells.

建立用于诊断水凝胶生成的癌症干细胞的拉曼显微镜新平台。

• 批准号: 22K18179
• 负责人: Clement Jean・Emmanuel
• 金额: $ 2.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K18179/
• 关键词: Raman imaging; Data standardadization; stemness markor; cell moprhology; molecular imaging; machine learning; Raman imaging; cancer stem cells

During this fiscal year was investigated the action of PAMPS gel on myoblast cell models. It was found that Poly (2-acrylamido-2- methyl-1-propanesulphonic acid) hydrogel have a significant impact to induce an elevation of stemness markers such as Sox2, Nanog, and Oct3/4. This elevation of stemness markers have been linked to a change of cell morphology, meaning that cell that have a higher expression level tend to be more round and small whereas the one that have a low expression of stemness markers tend to be bigger and flat. This interesting fact showed that by stainingThe first Raman images of the mentioned hydrogel-cell interaction system was performed with a high-speed slit-scanning Raman microscope. The quality of the measurement was performed with different metric such as signal-to-noise ratio, Laplacian operator and non-homogenous line illumination quantification. As a result the measurement protocol for this complex measurement provide high-quality data.To exploit Hyperspectral Raman images on hydrogel substrate, two algorithms that allow to compare Data from different Raman devices and different dates of measurement was finalized. One algorithm is for single cell measurement. Another one is for 3D colony formation measurement.

在本财政年度，研究了PAMPS凝胶对成肌细胞模型的作用。研究发现，聚(2-丙烯酰胺-2-甲基-1-丙磺酸)水凝胶对诱导SOX2、Nanog和Oct3/4等茎标记的升高有显著影响。这种茎标记的升高与细胞形态的变化有关，这意味着表达水平高的细胞往往更圆更小，而茎标记表达低的细胞往往更大更平。这一有趣的事实表明，通过染色，上述水凝胶-细胞相互作用体系的第一个拉曼图像是用高速狭缝扫描拉曼显微镜进行的。测量质量通过信噪比、拉普拉斯算子和非均匀线照度量化等不同的指标进行。为了利用水凝胶衬底上的高光谱拉曼图像，完成了两种算法，允许比较来自不同拉曼设备和不同测量日期的数据。一种算法是用于单细胞测量。另一种是用于三维集落形成的测量。

项目成果

Gaussian Weighted Background Correction for Raman images : cancer stem cell detection

拉曼图像的高斯加权背景校正：癌症干细胞检测

• 发表时间: 2022
• 作者: Jean-Emmanuel Clement;Zannatul Ferdous,Masami Tsuda;Shinya Tanaka;Jian Ping Gong;Katsumasa Fujita;Tamiki Komatsuzaki
• 通讯作者: Tamiki Komatsuzaki

Establishment of a novel platform of Raman microscope for diagnosis of hydrogel-generated cancer stem cells.

建立用于诊断水凝胶生成的癌症干细胞的拉曼显微镜新平台。

• 发表时间: 2022
• 作者: Jean-Emmanuel Clement;Zannatul Ferdous,Masami Tsuda;Shinya Tanaka;Jian Ping Gong;Katsumasa Fujita;Tamiki Komatsuzaki
• 通讯作者: Tamiki Komatsuzaki