Point-of-care therapeutic monitoring of anti-bacterial using low-cost disposable sensor

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Monitoring the levels of antimicrobial drugs is crucial to avoid toxic effects. Therapeutic drug monitoring (TDM) is a helpful tool to achieve this. We have developed a vancomycin (VCM) sensor to monitor drug concentration in whole blood. However, current sensor stability and VCM sensitivity depend on chip base structure and amperometric measurement mode. Previously, we had two major problems: the molecularly imprinted polymer-grafted-carbon paste (MIP-CP) was not reproducible, and the chip sensor lost sensitivity within three days of packing. After several optimizations, we have successfully made a highly reproducible MIP-CP. This chip has good batch reproducibility and good long-term stability. Also, the long-term stability of the packed chip has improved to two weeks in buffer saline and ten days in whole bovine blood. This indicates a good improvement over the past chip sensor. The data has been successfully disseminated through three conferences.