Role of heterogeneous pyramidal cell populations and specific connectivity motifs in pattern completion and replay of memory sequences (A01)

海马尖锐波/纹波复合体(SWRs)是发生在海马CA3区的短时(50-100ms)协调网络活动。它们调节外显记忆的巩固，而它们的破坏会损害记忆，而它们的延长则会增强记忆。然而，这些事件发生的机制仍然不清楚。为了更好地了解SWR动力学，我们将专注于异质锥体细胞群和特定连接基序在记忆序列的模式完成和重播中的作用。我们将分析在SWR过程中CA3金字塔的差异嵌入，评估其特定的连通性，利用光遗传学在体外和体内操纵神经元亚群，并开发可数值模拟和数学分析的SWR的计算网络模型。这项工作将极大地扩展我们对锐波模型的理解，这些模型是我们在上一个资助期的联合实验-理论工作中建立的。

Hippocampal sharp wave/ripple complexes (SWRs) are short (50–100 ms) episodes of coordinated network activity arising in the hippocampal CA3 area. They mediate the consolidation of explicit memories, and their disruption impairs memory whereas their prolongation enhances memory. However, the mechanisms governing the occurrence of these episodes remain obscure. To better understand SWR dynamics, we will focus on the role of heterogeneous pyramidal cell populations and specific connectivity motifs in pattern completion and replay of memory sequences. We will analyze the differential embedding of CA3 pyramids during SWRs, assess their specific connectivity, manipulate subsets of neurons using optogenetics in vitro and in vivo, and develop computational network models of SWRs that can be numerically simulated and analyzed mathematically. This work will considerably extend our understanding of sharp-wave models, which were established in our joint experimental-theoretical work in the previous funding period.