DEVELOPMENT OF SPECIFIC ASSAY FOR MEASURING ACTIVE FREE FORM OF thyroid hormones for monitering thyroid function

开发用于测量活性游离形式甲状腺激素的特异性测定法以监测甲状腺功能

• 批准号: 61870108
• 负责人: MIYAI Ikiyoshi
• 金额: $ 2.3万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61870108/
• 关键词: enzyme immunoassay; radioimmunoassy; free thyroxine; 遊離型トリヨードサイロニン; 酵素免疫測定法; 新生児

New enzyme immunoassay (EIA) and radfioimmunoassay (RIA) for measuring free thyroxind (FI_4) and free triiodothyronine (FT_3) unifluenced by albumin and T_4 binding blobulin (TBG) were developed.(1) EIA of FT_4 A double-antibody EIA for determination of FT_4 in serum and dried blood samples on filter paper was developed with use of a T_4-<beta>-D-galactosidase complex. The measurable range of FT_4 concentration in two 3-mm blood discs, was 1.9 to 93 ng/L. The mean coefficients of variation (CV) were 7.6%(within assay) and 6.4%(berween assays.) Results for FT_4 by this method correlated well with those for serum determined by RIA. (2) RIA of FT_4 A double-antibody solid phase RIA for FT_4 in serum with use of^<125> I-labelled T_4-hcg conjugate was developed. Since the labelled conjutage does not bind to TBG and albumin, the method is uninfuenced by TBG or albumin. The measurable of FT_4 serum was 2.0 to 128 ng/l. The mean CV within and between assays were 4.6-8.6% and 6.3-11.6%, respect … More ively. The FT_4 concentrations is serum as determined by this method were 9 to 17 ng/l for healthy adult subjects; high for pationts with hyperthyroidism; low for patients with hypothroidism; and within normal limits for pregnant women, and parients tiwh high or low concentrations of TBG. (3) EIA of FT_3 In the new solid-phase antibody EIA for FT_3 in serum, <beta>-D-galactosidase conjugated to T_3 is use. Results are uninfulenced by physiological concentrations og TBG or albumin, Results correlate well with those determined by equilibrum dialysis. The mean CVs within and between assays were 6.1 and 9.5%, respectively. The measurable range of FT_3 in serum is 0.7 to 26 ng/L; the normal refernce interval is 1.9 to 8.9 nb/L. Concentrations of FT_3 in serum of patients with hyperthyoidism were high; those of patients with hypothyroidism were within normal limits or low, and those of patients with congenitally decteased ot increased TBG were within the normal range. These methods are useful for monitoring thyroid function. Less

本文建立了测定游离甲状腺素（FI_4）和游离三碘甲状腺原氨酸（FT_3）的酶免疫分析法（EIA）和放射免疫分析法（RIA）。(1)FT_4的酶免疫分析法（EIA）应用T_4-D-半乳糖苷酶复合物，建立了测定血清和滤纸干血中FT_4的双抗体酶免疫分析法。<beta>在两个3mm血片中，FT_4浓度可测范围为1.9~93ng/L。平均变异系数（CV）为7.6%（试验内）和6.4%（试验间）。本法测定血清FT_4的结果与放射免疫分析法测定结果一致。(2)本文报道了用碘标记的T_4-hCG偶联物，建立了测定血清FT_4的双抗体固相放射免疫分析法。<125>由于标记的缀合物不与TBG和白蛋白结合，因此该方法不受TBG或白蛋白的影响。血清FT_4测定值为2.0~128ng/l。试验内和试验间的平均CV分别为4.6 - 8.6%和6.3 - 11.6%， ...更多信息 ively。用本法测定的健康成人血清FT_4浓度为9~17ng/l，甲亢病人高，甲减病人低，孕妇及高、低TBG病人在正常范围内。(3)FT_3的酶联免疫测定法（EIA）是一种新的血清FT_3固相抗体EIA法，采用T_3标记-D-半乳糖苷酶。<beta>结果不受生理浓度TBG或白蛋白的影响，与平衡透析法测定结果相关性良好。试验内和试验间的平均CV分别为6.1%和9.5%。血清FT_3测定范围为0.7~26ng/L，正常值范围为1.9~8.9ng/L。甲亢患者血清FT_3浓度高，甲减患者血清FT_3浓度在正常范围内或偏低，先天性甲状腺功能减退或TBG增高患者血清FT_3浓度在正常范围内。这些方法对监测甲状腺功能很有用。少

项目成果

K. Miyai er al.: Plenum Medical Book Companr, New York. Measurement of "free thyroxine" in dried blood samples on filter paper by radio-and enzyme-immunoassay for monitering thyroid function: In Frontiers in Thyroidology., 4 (1986)

K. Miyai 等人：Plenum Medical Book Companr，纽约。

K.Miyai et al.Ed G.Mederios Nelo,E Gaitan: "Measurement of "free thyroxine" in dried blood samples onfilter paper by radio-and enzyme-immunoassay for monitering thyroid function: In Frontiers in Thyroidology" Plenum Medical Book Company, New York, 4 (1986

K.Miyai 等人，Ed G.Mederios Nelo，E Gaitan：“通过放射和酶免疫测定法在滤纸上测量干燥血液样本中的“游离甲状腺素”以监测甲状腺功能：甲状腺学前沿”Plenum Medical Book Company，

N.Hata et al.: Clin.Chem.33. 172-176 (1987)

N.Hata 等人：Clin.Chem.33。

K. Miyai er al.: VCH Verlagsgesellschaft.thyroxine. In methods of Enzymatic Analysis., 13 (1986)

K. Miyai 等人：VCH Verlagsgesellschaft.thyroxine。

N. HATA, et al.: "Enzyme immunouassay of free triiodothronine in serum." Clin. Chem.33. 172-176 (1987)

N. HATA 等人：“血清中游离三碘甲状腺原氨酸的酶免疫分析。”