Studies on the inhibitors from plant pathogens produced by soil germs

1.从红壤土壤中分离到的铜绿假单胞菌S-7对尖孢镰刀菌FA-3的生长有抑制作用。土壤中的细菌用甲醇提取，提取液在薄层层析上展开，薄层层析在培养的尖孢霉培养的琼脂上进行。琼脂上清晰的条带显示了含有生长抑制物的薄层色谱条带。从透明带中分离得到3个抑制物，分别鉴定为半花菁、氯代菲和吩嗪-1-羧酸。浓度分别为100、200和200µg/ml时对尖孢镰刀菌有抑制作用。这些抑制活性的实际应用现在是由北海道农业实验站开发的。从田间土壤中分离到的洋葱假单胞菌B-17也对尖孢镰刀菌有抑制作用。最低抑菌浓度为3.13微克/毫升，并用吡咯硝胺进行了鉴定。另一株菌株RB425对立枯丝核菌和黄萎病菌有较强的抑制作用，其抑制物归属于假单胞菌、2-庚烯-3-甲基-4-喹啉和2-壬烯-3-甲基-4-喹啉。对根癌农杆菌、米氏棒杆菌、胡萝卜欧文氏菌、青枯假单胞菌和米黄单胞菌也有抑制作用。尤其是壬烯基伪烷表现出了广泛的活性。格雷维氏乳杆菌具有抑制脂肪过氧化生成的活性，并对其进行了分离。用体外分离的肝细胞测定ADP和NADPH对脂肪的过氧化作用，用TBA法测定过氧化脂肪。新合成的缓蚀剂的结构经波谱分析确定为1-乙酰氧基-2，4-二羟基-6-香叶基香叶基苯。

1. Pseudomonas aeruginosa S-7, separated from the soil of azuki-field inhibited the growth of Fusarium oxysporum FA-3. The germ from the soil was extracted with methanol and the extracts were developed on TLC which was laid on the agar with cultivated F. oxysporum and icnubated. The TLC bands containing growth inhibitors were showed by clear zones on the agar. Three inhibitors were isolated from the clear zones and they were identified with hemipiocyanine, chlororaphine and phenazine-1-carboxylic acid. They inhibited F. oxysporum with the concentration 100, 200 and 200mug/ml respectively. Practical application of these inhibition activity was now developed by the Agricultural Experimental Station, Hokkaido.2. Pseudomonas cepacia B-17 separated from the soil of the field also inhibited F. Oxysporum. The germ by the minimum concentration of 3.13mug/ml. The inhibitor was identified with pyrrolnitrine. Another strain RB425 strongly inhibited Rhizoctonia solani and Verticillium dahlial and inhibitors were assigned to pseudans, 2-hepteny1-3-methy1-4-quinolinol and 2-noneny1-3-methy1-4-quinolinol. They inhibited Agrobacterium tumefaciens, Corynebacterium michigenese, Erwinia carotovora, Pseudomonas solanocearum and Xanthomonas oryzae, too. The nonenyl pseudane showed broad activity, especially.3. Suillus grevillei showed the activity of inhibiting from the production of fat peroxide and the inhibitor was isolated. Activity was evaluated by the enzymatic peroxidation of fat under existence of ADP and NADPH using separated liver cell in vitro and peroxygenated fat was measured by TBA method. The structure of newly obtained inhibitor was determined as 1-acetoxy-2,4-dihydroxy-6-geranyl geranyl benzene by mainly spectroscopic methods.