Revised transformation of retrovirus-infected cells by N__--myristoyl peptide derivatives

新合成的N-肉豆蔻酰甘氨酸二乙缩醛(CH_3(CH_2)_&lt；11&gt；CH_2-CONHCH_2(CO_2H_5)_2，)和其他N-Myr-化合物被用于人类T细胞白血病病毒和人类免疫缺陷病毒结构蛋白的NH_2末端抗肌苷甲基化反应。用Rous肉瘤病毒(RSV)温度敏感突变体(TsNY68)感染鸡胚成纤维细胞(CEF)，结果表明，N-Myr-Goa对HTLV-I、HIV-I^1的p17^&lt；Gag&gt；和tsNY68的转化蛋白pp60^&lt；v-src&gt；的p19^&lt；gag&gt；蛋白的肉豆蔻酰化有极强的抑制作用。N-Myr-GoA还可阻止tsNY68感染的CEF的形态转化，而非肉豆蔻酰化的pp60；v-src&gt；可引起感染的CEF的反向转化。

N-myristoyl glycinal diethyacetal, CH_3(CH_2)_<11>CH_2-CONHCH_2(CO_2H_5)_2, which is abbreviated as N-MYr-GOA, and the other N-Myr-compounds were newly synthesized and then employed for a NH_2-terminal antimyristoylation of structure proteins in the human T-cell leukemia virus(HTLV-I) and the human immuno-deficiency virus(HIV). The compounds were also tested for reverse transformation of chick embryo fibroblast(CEF) infected with a temperature-sensitive mutant(tsNY68) of Rous sarcoma virus(RSV).of the compounds tested, N-Myr-GOA extremely inhibited protein myristoylation of p19^<gag> of HTLV-I, p17^<gag> of HIV-I^1, and pp60^<v-src>, which is a transforming protein of tsNY68. N-Myr-GOA also prevented morphological transformation of tsNY68-infected-CEF, and nonmyristoylated pp60^<v-src> caused reverse transformation of the infected-CEF.