Revised transformation of retrovirus-infected cells by N__--myristoyl peptide derivatives

N__--肉豆蔻酰肽衍生物对逆转录病毒感染细胞的改良转化

• 批准号: 62870095
• 负责人: KUBOTA Yukiho
• 金额: $ 2.24万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62870095/
• 关键词: Myristoyl peptide; retrovirus; human retroviruses; HTLV-I; HIV; レトロウイルス

N-myristoyl glycinal diethyacetal, CH_3(CH_2)_<11>CH_2-CONHCH_2(CO_2H_5)_2, which is abbreviated as N-MYr-GOA, and the other N-Myr-compounds were newly synthesized and then employed for a NH_2-terminal antimyristoylation of structure proteins in the human T-cell leukemia virus(HTLV-I) and the human immuno-deficiency virus(HIV). The compounds were also tested for reverse transformation of chick embryo fibroblast(CEF) infected with a temperature-sensitive mutant(tsNY68) of Rous sarcoma virus(RSV).of the compounds tested, N-Myr-GOA extremely inhibited protein myristoylation of p19^<gag> of HTLV-I, p17^<gag> of HIV-I^1, and pp60^<v-src>, which is a transforming protein of tsNY68. N-Myr-GOA also prevented morphological transformation of tsNY68-infected-CEF, and nonmyristoylated pp60^<v-src> caused reverse transformation of the infected-CEF.

合成了N-肉豆蔻酰甘氨酸二乙缩醛（CH_3（CH_2）_CH_2<11>-CONHCH_2（CO_2H_5）_2，简称N-MYr-果阿）及其它N-肉豆蔻酰甘氨酸二乙缩醛化合物。这些化合物还被测试用于感染劳斯肉瘤病毒（RSV）的温度敏感突变体（tsNY 68）的鸡胚成纤维细胞（CEF）的逆转。在测试的化合物中，N-Myr-果阿极大地抑制<gag>HTLV-1的p19^、<gag>HIV-1的p17^和<v-src>作为tsNY 68的转化蛋白的pp 60 ^的蛋白豆蔻酰化。N-Myr-果阿也阻止了tsNY 68感染的CEF的形态转化，而非肉豆蔻酰化的pp 60-γ<v-src>引起了感染的CEF的逆转。

项目成果

Shozo Shoji;Akira Tashiro;Fyuichi Tsujita;Manabu Matsanaga;Yukiho Kubota: Peptide Chemistry. 1988. 273-278 (1989)

Shozo Shoji；Akira Tashiro；Fyuichi Tsujita；Manabu Matsanaga；Yukiho Kubota：肽化学。

S. Shoji;A. Tashiro;R. Tsujita;M. Matsunaga;Y. Kubota: "Effect of N-Myristoyl Glycyl Peptide Derivatives on Protein Myristoylation of Structure Proteins and src-gene Product in Retrovirus-infected Cells" Peptide Chemistry. 1988. 273-278 (1989)

S.庄二；A.

Shozo Shoji;Akira Tashiro;Yukiho Kubota: Journal Biochemistry. 103. 747-749 (1988)

Shozo Shoji；Akira Tashiro；Yukiho Kubota：生物化学杂志。

Shozo Shoji;Akira Tashiro;Fyuichi Tsujita;Manabu Matsunaga;Yukiho Kubota: Peptide Chemistry. 1988. 273-278 (1989)

Shozo Shoji；Akira Tashiro；Fyuichi Tsujita；Manabu Matsunaga；Yukiho Kubota：肽化学。

Shozo Shoji;Yukiho Kubota: YAKUGAKU ZASSHI. 109(2). 71-85 (1989)

Shozo Shoji；Yukiho Kubota：药乐杂子。