Development of two wavelength excitation system for photodynamic therapy

光动力治疗双波长激发系统的开发

• 批准号: 62870098
• 负责人: NISHISAKA Tsuyoshi
• 金额: $ 4.03万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62870098/
• 关键词: photodynamic therapy; triplet state; マイクロプロセッサー; 三重項状態

The advantages of photosensitization process over photodynamic action has been desired. In this study we developed the two-step excitation system for photodynamic therapy using the CW laser and compared with the conventional one-step excitation method. The system consist of following components.1) Argon ion laser. 2) Microprocessor controlled variable beam splitter which splits the laser light. One light is for singlet state excitation and other for DCM dye laser pumping source. 3) Beam combiner which combine Argon and dye lasers light. 4) Collimating lenses for fiber optics. Output power of two laser light is monitored on CRT screen during the irradiation period.The cytocidal effect in HeLa cell was found to be higher than that of conventional method. Results obtained on the C3H/MH-134 solid tumor model seem to indicate a better tumor response as compared with that of conventional one. This systems can be optimized for any given sensitizers choosing suitable excitation wavelengths for the two step excitation.

光敏化过程相对于光动力作用的优点已经被期望。在本研究中，我们发展了两步激发系统的光动力治疗使用连续激光，并与传统的一步激发方法进行了比较。该系统由以下部分组成：1）氩离子激光器。2)微处理器控制的可变分束器将激光分开。一路光用于单重态激发，另一路光用于DCM染料激光泵浦源。3)联合收割机将氩激光和染料激光合束。4)光纤用准直透镜。在CRT显示屏上监测两种激光的输出功率，发现其对HeLa细胞的杀伤效果高于常规方法。在C3 H/MH-134实体瘤模型上获得的结果似乎表明与常规模型相比更好的肿瘤反应。该系统可以针对任何给定的敏化剂进行优化，为两步激发选择合适的激发波长。

项目成果

西坂剛: 医用電子と生体工学. 24(Suppl). 514 (1988)

西坂刚：医疗电子学和生物工程24（增刊）514（1988）。

西坂剛: 日本レーザー医学会誌. 8. 31-32 (1988)

Tsuyoshi Nishizaka：日本激光医学会杂志，8. 31-32 (1988)。

西坂剛: 日本レーザ ー医学会誌. 8. 31-32 (1988)

Tsuyoshi Nishizaka：日本激光医学会杂志，8. 31-32 (1988)。

西坂剛: 医用電子 と生体工学. 24. 514 (1988)

西坂刚：医疗电子学和生物工程。24. 514 (1988)。