Development of two wavelength excitation system for photodynamic therapy

与光动力作用相比，光敏化过程的优点已经被人们所期望。在这项研究中，我们开发了用于光动力疗法的两步激发系统，并与传统的一步激发方法进行了比较。该系统由以下部件组成：1)Ar离子激光器。2)微处理器控制的可变分束器，对激光进行分束。一束光用于单态激发，另一束光用于DCM染料激光泵浦。3)将氩激光和染料激光组合在一起的合束器。4)光纤准直透镜。在照射期间，在CRT屏幕上监测两束激光的输出功率，发现在HeLa细胞中的细胞杀伤率高于常规方法。在C3H/MH-134实体瘤模型上的结果似乎表明，与传统的肿瘤模型相比，肿瘤反应更好。该系统可以针对任何给定的敏化剂进行优化，为两步激励选择合适的激发波长。

The advantages of photosensitization process over photodynamic action has been desired. In this study we developed the two-step excitation system for photodynamic therapy using the CW laser and compared with the conventional one-step excitation method. The system consist of following components.1) Argon ion laser. 2) Microprocessor controlled variable beam splitter which splits the laser light. One light is for singlet state excitation and other for DCM dye laser pumping source. 3) Beam combiner which combine Argon and dye lasers light. 4) Collimating lenses for fiber optics. Output power of two laser light is monitored on CRT screen during the irradiation period.The cytocidal effect in HeLa cell was found to be higher than that of conventional method. Results obtained on the C3H/MH-134 solid tumor model seem to indicate a better tumor response as compared with that of conventional one. This systems can be optimized for any given sensitizers choosing suitable excitation wavelengths for the two step excitation.