Demonstration of the formation of cyclobutane pyrimidine dimers through the excited triplet state mediated by solar UV radiation

演示通过太阳紫外线辐射介导的激发三重态形成环丁烷嘧啶二聚体

• 批准号: 23651044
• 负责人: IKEHATA Hironobu
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651044/
• 关键词: 紫外線; 光生物学; 光化学; 環境; DNA損傷; 光化学反応

I tried to demonstrate that the formation of cyclobutane pyrimidine dimers (CPDs) by long wavelength UV radiations is mediated by a photochemical reaction through the excited triplet states of DNA bases. However, it was reported by others that the long wavelength UV-induced CPD formation is mediated by another novel mechanism, which made us change the goal of this research and characterize the novel mechanism by studying comparatively the mutation spectra induced by long wavelength UV. We found that solar-UV signature mutations, which we had identified before as solar UV-specific mutations, are caused through the CPDs produced by this novel mechanism, and are induced most efficiently by UVA1.

我试图证明，形成环丁烷嘧啶二聚体（CPD）的长波长紫外线辐射介导的光化学反应通过激发三重态的DNA碱基。然而，其他人报道长波长UV诱导CPD形成是由另一种新的机制介导的，这使得我们改变了本研究的目标，并通过比较长波长UV诱导的突变光谱来表征新的机制。我们发现，我们之前确定为太阳紫外线特异性突变的太阳紫外线特征突变是通过这种新机制产生的CPD引起的，并且是由UVA 1最有效地诱导的。

项目成果

Solar-UV-signature mutation prefers TCG to CCG: extrapolative consideration from UVA1-induced mutation spectra in mouse skin

• DOI: 10.1039/c3pp25444e
• 发表时间: 2013-01-01
• 期刊: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
• 影响因子: 3.1
• 作者: Ikehata, Hironobu;Kumagai, Jun;Morita, Akimichi
• 通讯作者: Morita, Akimichi

皮膚における紫外線に対する変異誘発抑制応答と紅斑反応の関連

皮肤对紫外线的致突变抑制反应与红斑反应的关系

• 发表时间: 2012
• 作者: 池畑広伸;東正一;亀井保博
• 通讯作者: 亀井保博

Risk estimation of the solar-UV genotoxicity...

太阳紫外线遗传毒性的风险评估...

• 发表时间: 2011
• 作者: Takaku;M.;Tsujita;T.;Horikoshi;N.;Takizawa;Y.;Qing;Y.;Hirota;K.;Ikura;M.;Ikura;T.;Takeda;S.;Kurumizaka;H.;H. Ikehata
• 通讯作者: H. Ikehata

Fully functional global genome repair of (6-4) photoproducts and compromised transcription-coupled repair of cyclobutane pyrimidine dimers in condensed mitotic chromatin

• DOI: 10.1016/j.yexcr.2012.01.003
• 发表时间: 2012-03-10
• 期刊: EXPERIMENTAL CELL RESEARCH
• 影响因子: 3.7
• 作者: Komura, Jun-ichiro;Ikehata, Hironobu;Ono, Tetsuya
• 通讯作者: Ono, Tetsuya

M期の凝縮した染色体におけるDNA修復:効率的なゲノム全体の除去修復と不活性な転写共役修復

M期浓缩染色体中的DNA修复：高效的全基因组切除修复和失活转录偶联修复

• 发表时间: 2011
• 作者: 小村潤一郎;池畑広伸;森俊雄;小野哲也
• 通讯作者: 小野哲也