Artificial Organ and Complement----Basic Studies for Development of New Biomedical Polymer

人造器官及其补体——新型生物医用高分子材料开发的基础研究。人工合成高分子材料表面补体系统的活化一直是人工器官发展的一个重要问题。在本研究中，我们重点建立了一种简单的补体活化测定方法，并利用所建立的方法对各种用于血浆分离器的人工膜进行了评价。1)制备荧光标记的人补体成分抗体。鉴定结合在人工膜上的补体成分似乎为补体被人工膜激活的机制提供了有用的信息。因此，我们制备了十种人体补体成分的抗体，并用荧光试剂FITC对其进行标记。发现FITC抗体可用于鉴定特发性膜性肾小球肾炎沉积的补体成分。2)补体片段新抗原特异性单克隆抗体的制备。我们制备了四种针对补体成分活化后新出现抗原的单克隆抗体。利用这些单克隆抗体，我们建立了测定人工膜活化补体系统的简单方法。3)等离子分离器用膜的评价。北海道红十字会血液中心一直在尝试建立一种利用朝日医药公司生产的膜分离浆细胞的新系统。我们已经评估了血液与膜分离后补体系统的激活情况，并为开发实用的膜积累了信息。

Artificial organ and complement-Basic studies for development of new biomedical polymers.Activation of complement system at synthetic polymer surface has been a serious problem upon development of artificial organs.In this study, we have focused to establish a simple method for determination of complement activation and utilizing the established method to evaluate various artificial membranes prepared for the purpose of plasma separator.1) Preparation of fluorescence-labeled antibodies to human complement components. Identification of complement components bound on artificial membranesd seems to provide useful informations for the mechanism of complement activation by the artificial membranes. So, we have prepared antibodies to ten human complement components and labeled them with a fluorescent reagent, FITC. The FITC antibodies were found to be useful for identification of complement components deposited on idiopathic membranous glomerulonephritis.2) Preparation of monoclonal antibodies specific for neoantigens of complement fragments. We have prepared four monoclonal antibodies specific for the newly appeared antigen upon activation of complement components. Utilizing these monoclonal antibodies , we have established simple methods for determination of activation of complement system by artificial membranes.3) Assessment of membranes for the plasma separator. The Blood Center of Hokkaido Red cross has been attempting to establish a new system for plasma-cell separation by using membranes, which were produced by Asahi Medica Co. We have assessed the activation of complement system after separation of blood with the membranes and have been accumulating informations for the development of practical membranes for this purpose.