Artificial Organ and Complement----Basic Studies for Development of New Biomedical Polymer

人工器官和补体-开发新型生物医学聚合物的基础研究。人工器官表面补体系统的激活一直是人工器官发展的一个严重问题。在本研究中，我们重点建立了一种简单的测定补体激活的方法，并利用所建立的方法来评价各种用于血浆分离器的人工膜1)制备针对人类补体成分的荧光标记抗体。人工膜结合补体成分的鉴定似乎为人工膜激活补体的机制提供了有用的信息。因此，我们制备了针对10种人类补体成分的抗体，并用荧光试剂FITC标记它们。FITC抗体可用于鉴定沉积在特发性膜性肾小球肾炎的补体成分。2)制备针对补体片段新抗原的单抗。我们已经制备了四种针对补体成分激活后新出现的抗原的单抗。利用这些单抗，我们建立了测定人工膜激活补体系统的简单方法。3)血浆分离器膜的评估。北海道红十字会血液中心一直在尝试建立一种利用朝日药品株式会社生产的膜分离血浆-细胞的新系统。我们评估了用膜分离血液后补体系统的激活情况，并为开发实用的膜积累了信息。

Artificial organ and complement-Basic studies for development of new biomedical polymers.Activation of complement system at synthetic polymer surface has been a serious problem upon development of artificial organs.In this study, we have focused to establish a simple method for determination of complement activation and utilizing the established method to evaluate various artificial membranes prepared for the purpose of plasma separator.1) Preparation of fluorescence-labeled antibodies to human complement components. Identification of complement components bound on artificial membranesd seems to provide useful informations for the mechanism of complement activation by the artificial membranes. So, we have prepared antibodies to ten human complement components and labeled them with a fluorescent reagent, FITC. The FITC antibodies were found to be useful for identification of complement components deposited on idiopathic membranous glomerulonephritis.2) Preparation of monoclonal antibodies specific for neoantigens of complement fragments. We have prepared four monoclonal antibodies specific for the newly appeared antigen upon activation of complement components. Utilizing these monoclonal antibodies , we have established simple methods for determination of activation of complement system by artificial membranes.3) Assessment of membranes for the plasma separator. The Blood Center of Hokkaido Red cross has been attempting to establish a new system for plasma-cell separation by using membranes, which were produced by Asahi Medica Co. We have assessed the activation of complement system after separation of blood with the membranes and have been accumulating informations for the development of practical membranes for this purpose.