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Artificial Organ and Complement----Basic Studies for Development of New Biomedical Polymer

人工器官及补体----新型生物医用高分子材料开发的基础研究

基本信息

批准号：
62870104
负责人：
NAGASAWA Shigeharu
金额：
$ 3.01万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62870104/
关键词：
Artificial Organ Medical Polymer Complement 血漿分離膜

项目摘要

Artificial organ and complement-Basic studies for development of new biomedical polymers.Activation of complement system at synthetic polymer surface has been a serious problem upon development of artificial organs.In this study, we have focused to establish a simple method for determination of complement activation and utilizing the established method to evaluate various artificial membranes prepared for the purpose of plasma separator.1) Preparation of fluorescence-labeled antibodies to human complement components. Identification of complement components bound on artificial membranesd seems to provide useful informations for the mechanism of complement activation by the artificial membranes. So, we have prepared antibodies to ten human complement components and labeled them with a fluorescent reagent, FITC. The FITC antibodies were found to be useful for identification of complement components deposited on idiopathic membranous glomerulonephritis.2) Preparation of monoclonal antibodies specific for neoantigens of complement fragments. We have prepared four monoclonal antibodies specific for the newly appeared antigen upon activation of complement components. Utilizing these monoclonal antibodies , we have established simple methods for determination of activation of complement system by artificial membranes.3) Assessment of membranes for the plasma separator. The Blood Center of Hokkaido Red cross has been attempting to establish a new system for plasma-cell separation by using membranes, which were produced by Asahi Medica Co. We have assessed the activation of complement system after separation of blood with the membranes and have been accumulating informations for the development of practical membranes for this purpose.

人工器官和补体--开发新型生物医用高分子的基础研究。在人工器官开发过程中，人工高分子表面补体系统的活化一直是一个严重的问题。在本研究中，我们致力于建立一种用于测定补体激活的简单方法，并利用所建立的方法来评价用于血浆分离器目的的各种人工膜。人补体成分的标记抗体。对人工膜上结合的补体成分的鉴定，为人工膜激活补体的机制提供了有用的信息。因此，我们制备了针对10种人类补体成分的抗体，并用荧光试剂FITC标记它们。FITC抗体可用于鉴别特发性膜性肾小球肾炎沉积的补体成分。2）补体片段新抗原特异性单克隆抗体的制备。我们已经制备了四种特异性单克隆抗体的新出现的抗原激活后的补体成分。利用这些单克隆抗体，我们建立了测定人工膜激活补体系统的简便方法。3）血浆分离器膜的评价。北海道红十字会血液中心一直在尝试使用Asahi Medica Co.生产的膜建立一种新的浆细胞分离系统。我们评估了使用该膜分离血液后补体系统的活化，并为开发用于此目的的实用膜积累了信息。