Studies on molecular constitution and effector functions of complement system
补体系统分子构成及效应功能研究
基本信息
- 批准号:04454527
- 负责人:
- 金额:$ 4.1万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1992
- 资助国家:日本
- 起止时间:1992 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.A conformational change in C4 : Using monoclonal antibody against a neoantigen in C4, we have determined a specific hydrophobic domain in C4, which becomes exposed from interior portion to the surface upon activation of C4 by C1s.2.Structure and functions of regulatory factors of complement system : We have compared the regulatory effects of MCP, DAF, and MCP-DAF hybrid molecule against human complement by expressing the factors on Chinese hamster ovary cells. Hybrid molecule exerted additive protective effect against the alternative pathway of complement but was less potent in the classical pathway than DAF alone.3.Histamine-induced expression of DAF on human umbilical vein endothelial cells (HUVEC) : DAF expression on HUVEC was found to be enhanced about 2 fold with 10 mM histamine through H_1 receptor. These results suggest that histamine, which is released from mast cells and basophils by C5a anaphylatoxin, increases the complement defense ability of endothelial cells by increaseing their levels of DAF expression.4.Biological functions and receptors of anaphylatoxins, C3a and C4a. We have revealed the presence of two types of C3a receptor on guinea pig macrophages and found that C3a receptor-mediated cellular responses are down-regulated by C4a. These results suggest that contrary to general assumption, C4a receptor is functionally different from C3a receptor.
1.C4的构象变化:利用针对C4中新抗原的单克隆抗体,我们确定了C4中特定的疏水结构域,该结构域在C4被cs1激活后从内部部分暴露到表面。补体系统调节因子的结构和功能:我们通过在中国仓鼠卵巢细胞上表达MCP、DAF和MCP-DAF杂交分子对人补体的调节作用进行了比较。杂化分子对补体替代途径具有加性保护作用,但对经典途径的保护作用不如单用DAF。组胺诱导DAF在人脐静脉内皮细胞(HUVEC)上的表达:10 mM组胺通过H_1受体使HUVEC上DAF的表达增强约2倍。这些结果表明,C5a过敏毒素从肥大细胞和嗜碱性细胞释放的组胺通过提高内皮细胞DAF的表达水平来增强内皮细胞的补体防御能力。过敏毒素C3a和C4a的生物学功能和受体。我们发现豚鼠巨噬细胞中存在两种类型的C3a受体,发现C3a受体介导的细胞反应被C4a下调。这些结果表明,与一般假设相反,C4a受体与C3a受体在功能上是不同的。
项目成果
期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Maeda,S.: "Identification of a surface structure in the fourth component of human complement,C4,which becomes hidden upon activation by C1s." Biochem.J.289. 503-508 (1993)
Maeda,S.:“鉴定了人类补体第四种成分 C4 的表面结构,该结构在被 C1 激活后会被隐藏。”
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Tsuji,S.: "Decay-accelerating factor on human umbilical vein endothelial cells.Its histamine-induced expression and spontaneous rapid shedding from the cell surface." J.Immunol.153. (1994)
Tsuji,S.:“人脐静脉内皮细胞的衰变加速因子。其组胺诱导表达并从细胞表面自发快速脱落。”
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- 影响因子:0
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Murakami,Y.: "Cellular responses guinea-pig macrophages to C4a:Inhibition of C3a-induced O2┣D1-┫D1┫D1┣D22┣D1-┫D1 generation " Immunol.Lett.36. 301-304 (1993)
Murakami, Y.:“豚鼠巨噬细胞对 C4a 的细胞反应:C3a 诱导的 O2┣D1-┫D1┫D1┣D22┣D1-┫D1 一代的抑制”Immunol.Lett.301-304(1993)。
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Tsuji, S., Kaji, K., & Nagasawa, S.: "Decay accelerating factor on human umbilical vein endothelial cells. Its histamine-induced expression and spontaneous rapid shedding from the cell sruface." J.Immunol. 153.
辻,S.,梶,K.,
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Murakami,Y.: "Characterization of C3a anaphylatoxin receptor on guinea-pig macrophages." Immunology. 79. 633-638 (1993)
Murakami,Y.:“豚鼠巨噬细胞上 C3a 过敏毒素受体的表征。”
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NAGASAWA Shigeharu其他文献
NAGASAWA Shigeharu的其他文献
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{{ truncateString('NAGASAWA Shigeharu', 18)}}的其他基金
Studies on effector molecules of innate immunity responsible for cytotoxicity to tumor cells.
对负责肿瘤细胞细胞毒性的先天免疫效应分子的研究。
- 批准号:
10470478 - 财政年份:1998
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
APOPTOSIS AND COMPLEMENT-STUDIES ON THE MECHANISM OF COMPKEMENT ACTIVATION AND THE BIOLOGICAL SIGNIFICANCE
细胞凋亡与补体——补体激活机制及其生物学意义的研究
- 批准号:
08457601 - 财政年份:1996
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
STUDIES ON THE BIOLOGICAL FUNCTIONS OF THE COMPLEMENT SYSTEM
补体系统生物学功能的研究
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06454594 - 财政年份:1994
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$ 4.1万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Biochemical Studies on Human Inter- trypsin Inhibitor
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62580106 - 财政年份:1987
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$ 4.1万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Artificial Organ and Complement----Basic Studies for Development of New Biomedical Polymer
人工器官及补体----新型生物医用高分子材料开发的基础研究
- 批准号:
62870104 - 财政年份:1987
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
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