Loss of the MG-induced cellular defense mechanism leads to the development of diabetic complications (C07+)

MG 诱导的细胞防御机制的丧失会导致糖尿病并发症的发生 (C07)

• 批准号: 408084766
• 金额: --
• 依托单位: Klinik für Endokrinologie, Diabetologie, Stoffwechselkrankheiten und Klinische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/408084766?language=en
• 关键词: Loss; MG; induced; cellular; defense

Aim is the identification of the sensors, mediators and effectors of the MG-induced defense response. Mouse endothelial cells will be preconditioned using MG and HG, followed by characterization via mRNASeq, (phospho-)proteome analysis and endothelial function assays. Next, the role of the previously identified effector Hsp70 will be studied in regard to MG-induced protein aggregation, also by using Hsp70-knockout mice. The last part will focus on the identification of potential biomarkers to help identify patients at high risk for the development of diabetic late complications.

目的是识别 MG 诱导的防御反应的传感器、介体和效应器。小鼠内皮细胞将使用 MG 和 HG 进行预处理，然后通过 mRNASeq、（磷酸）蛋白质组分析和内皮功能测定进行表征。接下来，将通过使用 Hsp70 敲除小鼠来研究先前确定的效应子 Hsp70 在 MG 诱导的蛋白质聚集方面的作用。最后一部分将重点关注潜在生物标志物的识别，以帮助识别糖尿病晚期并发症发生高风险的患者。