Biotic and abiotic factors that dive the function of microbial communities at biogeochemical interfaces in different soils (BAMISO)

生物地球化学界面塑造土壤微生物群落功能。另一方面，微生物群落影响生态地球化学界面的性质。尽管这种相互作用的重要性，微生物性能的生物地球化学界面的作用的基本理解仍然缺失。我们假设，土壤中的微生物地球化学界面是重要的功能财团的微生物，这是能够塑造自己的微环境，从而影响土壤中的界面的属性的形成。此外，生物地球化学界面还可以作为土壤的遗传记忆，因为它们可以储存死亡微生物的DNA，并保护其免受降解。我们认为，对于功能性生物地球化学界面的形成，（例如，沿着真菌网络）响应生物可利用的碳和/或水可用性的质量和数量起着主要作用，因为微生物的功能性公会的发展需要能量并且取决于栖息地的氧化还原状态。将在不同发育的人工和自然土壤中研究十六烷降解情况。为了回答碳的数量和质量的作用的问题，将进行实验，有和没有凋落物材料在不同的土壤含水量。实验将与完整的土柱以及土壤样品中开发的界面结构已被人为破坏。十六烷降解微生物群落的分子分析将在体外和原位进行。在优先计划的第一阶段，已成功开发了相应的工具箱，包括基因组、转录组和蛋白质组分析方法。

Biogeochemical interfaces shape microbial community function in soil. On the other hand microbial communities influence the properties of biogeochemical interfaces. Despite the importance of this interplay, basic understanding of the role of biogeochemical interfaces for microbial performance is still missing. We postulate that biogeochemical interfaces in soil are important for the formation of functional consortia of microorganisms, which are able to shape their own microenvironment and therefore influence the properties of interfaces in soil. Furthermore biogeochemical interfaces act as genetic memory of soils, as they can store DNA from dead microbes and protect it from degradation. We propose that for the formation of functional biogeochemical interfaces microbial dispersal (e.g. along fungal networks) in response to quality and quantity of bioavailable carbon and/or water availability plays a major role, as the development of functional guilds of microbes requires energy and depends on the redox state of the habitat.To address these questions, hexadecane degradation will be studied in differently developed artificial and natural soils. To answer the question on the role of carbon quantity and quality, experiments will be performed with and without litter material at different water contents of the soil. Experiments will be performed with intact soil columns as well as soil samples where the developed interface structure has been artificially destroyed. Molecular analysis of hexadecane degrading microbial communties will be done in vitro as well as in situ. The corresponding toolbox has been successfully developed in the first phase of the priority program including methods for genome, transcriptome and proteome analysis.