Biotic and abiotic factors that dive the function of microbial communities at biogeochemical interfaces in different soils (BAMISO)

影响不同土壤生物地球化学界面微生物群落功能的生物和非生物因素（BAMISO）

• 批准号: 40874154
• 负责人: Professor Dr. Hauke Harms
• 金额: --
• 依托单位: Institut für Bodenökologie (IBOE) (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/40874154?language=en
• 关键词: Biotic; abiotic; factors; dive; function

Biogeochemical interfaces shape microbial community function in soil. On the other hand microbial communities influence the properties of biogeochemical interfaces. Despite the importance of this interplay, basic understanding of the role of biogeochemical interfaces for microbial performance is still missing. We postulate that biogeochemical interfaces in soil are important for the formation of functional consortia of microorganisms, which are able to shape their own microenvironment and therefore influence the properties of interfaces in soil. Furthermore biogeochemical interfaces act as genetic memory of soils, as they can store DNA from dead microbes and protect it from degradation. We propose that for the formation of functional biogeochemical interfaces microbial dispersal (e.g. along fungal networks) in response to quality and quantity of bioavailable carbon and/or water availability plays a major role, as the development of functional guilds of microbes requires energy and depends on the redox state of the habitat.To address these questions, hexadecane degradation will be studied in differently developed artificial and natural soils. To answer the question on the role of carbon quantity and quality, experiments will be performed with and without litter material at different water contents of the soil. Experiments will be performed with intact soil columns as well as soil samples where the developed interface structure has been artificially destroyed. Molecular analysis of hexadecane degrading microbial communties will be done in vitro as well as in situ. The corresponding toolbox has been successfully developed in the first phase of the priority program including methods for genome, transcriptome and proteome analysis.

生物地球化学界面影响土壤微生物群落功能。另一方面，微生物群落影响着生物地球化学界面的性质。尽管这种相互作用很重要，但对生物地球化学界面对微生物性能的作用的基本理解仍然缺失。我们假设土壤中的生物地球化学界面对于微生物功能群落的形成是重要的，微生物能够塑造自己的微环境，从而影响土壤中界面的性质。此外，生物地球化学界面充当土壤的遗传记忆，因为它们可以储存来自死亡微生物的DNA并保护其免受降解。我们认为，微生物的扩散（例如沿着真菌网络）对生物可利用碳和/或水可用性的质量和数量的响应在形成功能性生物地球化学界面方面起着重要作用，因为微生物的功能行业的发展需要能量，并取决于栖息地的氧化还原状态。为了解决这些问题，十六烷的降解将在不同开发的人工和自然土壤中进行研究。为了回答碳的数量和质量的作用问题，将在不同土壤含水量下进行有凋落物和没有凋落物的实验。实验将用完整的土柱进行，也将用人工破坏已发育的界面结构的土样品进行。十六烷降解微生物群落的分子分析将在体外和原位进行。相应的工具箱已在优先计划的第一阶段成功开发，包括基因组，转录组和蛋白质组分析方法。