Network Regulation of Expression and Activity of Binding Protein-Dependent ABC-Sugar Transport Systems of Thermus thermophilus

嗜热热菌是一种嗜热细菌，很可能是介于今天革兰氏阳性细菌和阴性细菌之间的进化产物（15）。在糖转运系统方面，它依赖于ABC转运体，但在古细菌中，它尚未获得糖磷酸转移酶系统（PTS）。我们将继续研究MalK的功能，这是三种ABC系统的共同运输atp酶。我们将根据转运活性的状态分析malk选择性服务于不同ABC转运体的机制。我们提出，在没有分解代谢抑制的情况下，碳利用的选择是通过控制MalK与相应渗透物的结合以及MalK调节结构域与参与糖代谢的蛋白质的特定相互作用来介导的。反之亦然，我们将遵循MalK的可能性，这取决于它在运输中的作用，将影响代谢途径。在这里，我们将重点介绍糖原的合成和降解。

Thermus thermophilus is a thermophilic bacterium that most likely represents an evolutionary intermediate between today's Gram positive and negative bacteria (15). In respect to sugar transport systems it relies on ABC transporters but as in archaea it has not yet aquired the sugar phosphotransferase system (PTS). We will proceed to study the function of MalK, the common transport ATPase for three ABC systems in T. thermophilus. We will analyse the mechanism of MalK-selectivity in serving the different ABC transporters dependent on the status of transport activity. We propose that in absence of catabolite repression the selection of carbon utilization is mediated by controlling the binding of MalK to the respective permeases and by the specific interaction of the regulatory domain of MalK with proteins involved in sugar metabolism. Vice versa we will follow the possibility that MalK, depending on its engagement in transport will affect metabolic pathways. Here we will focus on glycogen synthesis and degradation.