Molecular mechanisms of archaeal DNA replication: replication initiation and the replicative helicase MCM

古菌 DNA 复制的分子机制：复制起始和复制解旋酶 MCM

• 批准号: 41427621
• 负责人: Privatdozent Dr. Georg Lipps
• 金额: --
• 依托单位: Hochschule für Life Sciences FHNW
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/41427621?language=en
• 关键词: Molecular; mechanisms; archaeal; DNA; replication

The focus of this research proposal is the study of DNA replication of the thermophilic model archaeon Sulfolobus. We will follow two major approaches: first, study of the replication initiation by the Orc1/Cdc6 proteins and secondly characterization of the putative replicative helicase MCM. We will study the involved replication enzymes biochemically and analyze their enzymatic activities by a detailed analysis of their DNA binding properties, their enzymatic characteristics and most importantly how and whether they are engaged in larger protein complexes to fulfill their highly regulated and coordinated cellular functions at the origin and the replication fork, respectively. The proposed research will give us an insight into the machinery of initiation and replication on a molecular level and shall open up the avenue for structural as well as single-molecule studies of the thermostable and dynamic enzyme complexes involved. Since the archaeal DNA replication is similar to the eukaryal replication although less complex, the planned research will also help to deepen our understanding on the molecular mechanism of eukaryal DNA replication.

本研究计划的重点是研究嗜热模式古细菌Sulfolobus的DNA复制。我们将遵循两种主要方法：第一，研究Orc1/Cdc6蛋白的复制起始，第二，表征假定的复制解旋酶MCM。我们将通过详细分析其DNA结合特性、酶特性以及最重要的是它们如何以及是否参与更大的蛋白质复合物以分别在起点和复制叉处完成高度调节和协调的细胞功能来研究所涉及的复制酶的生化和分析其酶活性。所提出的研究将使我们对分子水平上的起始和复制机制有一个深入的了解，并将为所涉及的耐热性和动态酶复合物的结构和单分子研究开辟道路。由于古细菌的DNA复制与真核复制相似，但不那么复杂，因此计划的研究也将有助于加深我们对真核DNA复制的分子机制的理解。