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頭頸部癌の放射線治療効果予測のための分子生物学的研究

预测头颈癌放射治疗效果的分子生物学研究

基本信息

批准号：
08770701
负责人：
西岡健
金额：
$ 0.51万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08770701/
关键词：
頭頸部腫瘍 P53 放射線治療 yeast assay

项目摘要

1.対象と方法頭頸部扁平上皮癌37例(1991-)についてP53変異の有無を調べた.このうち17例が根治照射を,20例は術前照射を受けた.平均観察期間は17カ月であった.P53変異の検出はPCR-SSCP法を34例に用い,yeast assayを5例に用いた(2例では両方). yeast assayは,sampleのP53 DNAを酵母のDNAに導入し,sample由来のP53が機能するか否か(mutationがあるかないか)を酵母の色の変化で検出するという比較的簡便な方法である(A simple p53 functional assey for screening cell lines, blood, and tumors. Flamen et al. Proc Natl Acad Sci USA 92 : 3963-3967, 1995).照射効果は術前照射例でも評価可能とするため40Gy/16回時点の縮小率(腫瘍2方向計測値の積)で検討し4段階に評価した(very good≧75%, good 75-50%, moderate 50-25%, poor 25%≦).2、結果37例中12例(32.4%)に変異が認められた.原発部位,T stage,分化度と変異率の間には明らかな関係は認めなかった.P53変異の有無と40Gy時点での腫瘍の縮小率はP53の変異がない12症例ではvery good 9%, good 46%, moderate 36%, poor 9%であり変異があった25症例ではそれぞれ26%,17%,52%,4%であった.極めて良好な縮小(very good)を示した割合が,変異のない群で高い傾向がみられた.局所再発は変異が無い25症例中13例にみられ,変異があった12例中2例に認めた。3、結論P53の変異は局所再発のrisk factorとはいえず、変異が無いことがrisk factorとも言える結果であった。

1. Methods 37 cases of squamous cell carcinoma of the head and neck (1991-) were studied. 17 cases received radical irradiation and 20 cases received preoperative irradiation. PCR-SSCP method was used in 34 cases,yeast assay in 5 cases (2 cases). yeast assay A simple p53 functional assay for screening cell lines, blood, and tumors. Flamen et al. Proc Natl Acad Sci USA 92 : 3963-3967, 1995). The results of irradiation were compared with those of preoperative irradiation. The reduction rate (product of measurement values in 2 directions of tumor) at 40Gy/16 cycles was evaluated at 4 levels (very good ≥ 75%, good 75- 50%, moderate 50- 25%, poor 25% ≤). The original site,T stage, differentiation degree and difference rate were different between the two groups. P53 was different between the presence and absence of 40Gy. The reduction rate of P53 was very good 9%, good 46%, moderate 36%, poor 9% in 12 cases, 26%, 17%, 52%, 4% in 25 cases. Very good (very good), very different (very good), very different (very good). 13 of 25 cases were found to be abnormal and 2 of 12 cases were found to be abnormal. 3. Conclusion: The risk factor of P53 is different.