受精卵・着床前初期胚の培養環境と着床率の向上を目的としたヒト卵管上皮細胞株の樹立

建立人输卵管上皮细胞系，改善受精卵及早期着床前胚胎的培养环境及着床率

• 批准号: 08771321
• 负责人: 安藤 寿夫
• 金额: $ 0.7万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771321/
• 关键词: 初期胚; ヒト卵管; 細胞株

良性卵巣腫瘍に対する附属器摘除術や子宮筋腫に対する子宮全摘除術において、同意の下に得られた肉眼的に正常な生殖可能年齢の3名の患者由来の卵管を用いた。まず、酵素処理により細胞を分散し、コラーゲンI型をコートしたプラスチックディッシュ上へプレーティングした。培養開始後4日間は98%以上の純度で卵管上皮細胞の培養に成功し、以後線維芽細胞の割合が増加した。安定した株課細胞を得るためのSV40T抗原のトランスフェクションは培養3〜6日目のいずれかに行ない、8種類の細胞株を得た。このうち6種類の細胞株はサイトケラチンの産生を認め、卵管上皮由来と考えられた。2種類の細胞株はビメンチンの産生を認め、線維芽細胞由来と考えられた。現在、受精卵初期胚との共培養計に用いることのできるような安定した細胞株を得るために、これらの細胞株の継代を重ねている。

In benign ovaries, adnexectomy and total hysterectomy were performed in 3 patients with normal reproductive potential. The cell is divided into two parts: one part is divided into two parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part is divided into four parts: one part is divided into three parts: one part 4 days after culture, the purity of oocyte epithelial cells was more than 98%. The SV40T antigen was isolated from the stable cells and cultured for 3 ~ 6 days. 6 kinds of cell lines were identified, and the origin of ovum epithelium was examined. 2. The origin and development of cell lines and germ cells. Now, the co-culture of embryos at the early stage of fertilization is planned to be carried out in a stable cell line.

项目成果

HIROOKA,T.: "Synthesis and release of glycosylated prolactin in transfected cells" Endocrine J. 43. 423-428 (1996)

HIROKA,T.：“转染细胞中糖基化催乳素的合成和释放”Endocrine J. 43. 423-428 (1996)

後藤節子: "テキスト母性看護II" 名古屋大学出版会, 347 (1996)

后藤节子：《产妇护理文本II》名古屋大学出版社，347（1996）