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運動中の肝グリコーゲン分解におよぼす肝免疫細胞の影響

肝脏免疫细胞对运动过程中肝糖原降解的影响

基本信息

批准号：
08780107
负责人：
矢野博己
金额：
$ 0.64万
依托单位：
Kawasaki University of Medical Welfare
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08780107/
关键词：
Kupffer細胞 macrophage glycogenolysis prostaglandin 塩化ガドリニウム Fischer344rat 肝glycogen 肝循環

项目摘要

急性運動負荷初期に生じる,骨格筋の糖利用に先行して起こる肝グリコーゲン分解亢進には,肝マクロファージであるKupffer細胞と肝実質細胞間のcellular communicationが関与するかどうかについて研究を継続している.今回は,肝グリコーゲン分解がマクロファージ機能抑制ラットの場合,抑制されるのかどうかについて,検討を行った.実験動物にはFischer344系雌性ラットを用い,Gadolinium chlorideを実験開始24時間前に尾静脈投与した.安静群,運動群に分類した.運動は,トレッドミルランニングを負荷した.運動終了後,腹大静脈および門脈より採血を行った.肝は脱血後摘出し,肝グリコーゲン含量を測定した.血糖値,乳酸値,血漿プロスタグランディンD2濃度を測定した.運動負荷による血糖値の上昇及び肝グリコーゲン分解の亢進は観察されたが,Gadolinium chloride投与による,運動時の血糖値上昇抑制,あるいは肝グリコーゲン分解抑制は観察されなかった.しかし血漿PGD2濃度の動態からは,ラットにおける肝グリコーゲン分解作用へのマクロファージ機能抑制の影響は明らかにされず,今後さらに詳細な検討が必要であると考えられた.また急性運動がKupffer細胞の貧食能に及ぼす影響について,Fischer344系雌性ラットを用い,ランニングを60分間負荷し検討を行った.運動開始直前に蛍光ラテックス粒子を尾静脈投与し,運動終了後,肝は脱血,摘出後,凍結切片を作成し,蛍光顕微鏡下で肝に蓄積したラテックス粒子数を測定した.その結果,肝単位面積当たりのラテックス粒子数は運動群の方が高く,Kupffer細胞のラテックス粒子取り込み能の亢進の可能性が示唆された.しかしそのメカニズムについては明確にされなかった.

In the early stage of acute exercise load, bone gluten sugar was used to start the decomposition of liver cancer cells in advance, and the decomposition of liver cancer cells was hyperactive. Liver tissue culture was used to study the expression of cellular communication in the cells of Kupffer cells and to study the effect of bone gluten on metabolism. This time, the liver will be able to restrain the combination of the drugs, the symptoms, the symptoms, and the effects. The animal Fischer344 is used by the female, and the Gadolinium chloride will be put into use 24 hours before the start of the day. Quiet groups, action groups are classified into categories. Please do not pay any attention to the situation. After the exercise, the abdomen was quiet to make sure that the blood was broken. The blood of the liver was removed after the blood was removed, and the content of the liver was determined. Blood sugar, lactate, blood sugar, blood sugar, lactic acid, blood sugar, Exercise-induced hyperglycemia, Gadolinium chloride administration, hyperglycemia, hyperglycemia, hyperglycemia The blood PGD2 is sensitive, the liver is broken down, the liver is broken down, and the machine is able to suppress the impact. In the future, you will need to check the necessary information. In the case of acute exercise, the energy consumption and energy consumption of the Kupffer cell, which is used in the female diet, Fischer344 is used in the female diet, and the cycle is not available within 60 minutes. Before the start of the exercise, light was injected into the tail of the particles. After the exercise, the liver was drained of blood. After the exercise was taken out, the liver was cut into slices, and the liver was stored under light microscope to determine the number of particles. The results showed that the number of particles in the liver was significantly higher than that in the control group, and that the possibility of increasing the energy level of Kupffer cells was significantly higher than that of the control group. Please make sure that you have a clear understanding of what is going on.