Highspeed 0.7 mm MAS probe for a 800 MHz solid state NMR spectrometer

适用于 800 MHz 固态 NMR 波谱仪的高速 0.7 mm MAS 探头

• 批准号: 418091302
• 金额: --
• 依托单位: Technische Universität München (TUM)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/418091302?language=en
• 关键词: Highspeed; 0.7; mm; MAS; probe

Technical progress in solid-state NMR spectroscopy under magic angle spinning (MAS) has enabled significant improvement in sensitivity and resolution over the past few years. This methodology is essential for high-resolution studies on samples that are only available in sub-milligram amounts and cannot be obtained in deuterated form. Examples of such systems are proteins that cannot be expressed in eukaryotic systems or membrane proteins which cannot be refolded. Proton-detected experiments on such samples only reach the necessary resolution if MAS rates in the range above 100 kHz can be reached. These techniques were established in the past 3-4 years and only very recently have NMR probes become commercially available that enable such experiments. The possibility to conduct experiments on such equipment opens up completely new perspectives to research groups at BNMRZ addressing biological questions with solid-state MAS NMR.

在过去的几年里，魔角旋转（MAS）固态核磁共振波谱技术的进步使得灵敏度和分辨率有了显著的提高。这种方法对于样品的高分辨率研究至关重要，因为样品只能以亚毫克的量获得，无法以氘化形式获得。这种系统的例子是不能在真核系统中表达的蛋白质或不能再折叠的膜蛋白。只有在MAS速率达到100 kHz以上的情况下，质子检测实验才能达到必要的分辨率。这些技术是在过去的3-4年建立的，直到最近才有核磁共振探针商业化，使此类实验成为可能。在这种设备上进行实验的可能性为BNMRZ的研究小组用固态MAS NMR解决生物学问题开辟了全新的视角。