Activation of the unfolded protein response of the endoplasmic reticulum (UPRER) by aberrant Ca2+ signals (A21*)

异常 Ca2 信号 (A21*) 激活内质网 (UPRER) 的未折叠蛋白反应

• 批准号: 418686501
• 金额: --
• 依托单位: Fachrichtung Medizinische Biochemie und Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/418686501?language=en
• 关键词: Activation; unfolded; protein; response; endoplasmic

The endoplasmic reticulum (ER) is a key organelle of protein folding, lipid metabolism, and Ca2+ homeostasis. These functions are intrinsically connected to each other. Perturbation with any of these functions can cause ER-stress and followed by an activation of the unfolded protein response (UPR). This project aims to identify the mechanism of UPR activation by aberrant Ca2+ Signals and to establish the molecular rules underlying signal interaction by UPR transducers. Using sophisticated protocols for the immuno-isolation of organelles and state-of-the-art lipidomics, the lipid fingerprints of a stressed ER and of aberrant Ca2+ signals shall be established with subcellular resolution.

内质网（ER）是蛋白质折叠、脂质代谢和Ca 2+稳态的关键细胞器。这些功能在本质上是相互关联的。任何这些功能的干扰都可能导致ER应激，随后激活未折叠蛋白反应（UPR）。本项目旨在通过研究钙离子信号的异常激活UPR的机制，并建立UPR信号相互作用的分子规律。使用复杂的细胞器免疫分离方案和最先进的脂质组学，应建立一个应激ER和异常Ca2+信号的脂质指纹与亚细胞分辨率。