Quantitative interactomics of specific types of DNA damage (01)

特定类型 DNA 损伤的定量相互作用组学 (01)

• 批准号: 418751096
• 金额: --
• 依托单位: Institut für molekulare Virologie und Zellbiologie (IMVZ)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/418751096?language=en
• 关键词: Quantitative; interactomics; specific; types; DNA

In this proposal, we will use quantitative high-resolution mass spectrometry to identify proteins re-cruited to a range of defined DNA lesions, such as 8-oxo-dG, cyclobutane pyrimidine dimers and intrastrand cisplatin-DNA adducts, in different cell types and species. Putative new candidates will be further characterised by established biochemical and cell biology techniques. With this approach, we aim to identify complex networks of proteins involved in repair of these DNA lesions and uncover similarities and differences in DNA damage repair in organisms ranging from yeast to humans.

在这项提案中，我们将使用定量高分辨率质谱学来鉴定在不同细胞类型和物种中重新出现在一系列明确的DNA损伤中的蛋白质，例如8-oxo-DG、环丁烷嘧啶二聚体和链内顺铂-DNA加合物。假定的新候选者将进一步以已建立的生化和细胞生物学技术为特征。通过这种方法，我们的目标是识别参与这些DNA损伤修复的复杂蛋白质网络，并揭示从酵母到人类等生物中DNA损伤修复的异同。