Quantitative time-resolved analysis of DSB repair in individual living cells (14)

在这个项目中，我们建议使用单个活细胞的定量时间分辨分析来研究细胞周期进程中内源性和诱导性DNA双链断裂的修复。为此，我们将结合荧光报告细胞系的活细胞成像与计算图像处理和统计分析。因此，修复和信号通路的药理学扰动将使我们能够从机制上深入了解修复途径选择的后果，特别是在细胞周期转变期间。我们将使用所得到的定量数据来建立双链断裂修复和细胞周期进展之间相互作用的数学模型。

In this project, we propose to investigate the repair of endogenous and induced DNA double-strand breaks during cell cycle progression using quantitative time-resolved analysis of individual living cells. To this end, we will combine live-cell imaging of fluorescent reporter cell lines with computation-al image processing and statistical analysis. Pharmacological perturbations of repair and signalling pathways will thus allow us to gain mechanistic insight into the consequences of repair pathways choices specifically during cell cycle transitions. We will use the resulting quantitative data to estab-lish mathematical models of the interplay between double-strand break repair and cell cycle progres-sion.