尿路上皮癌細胞と、血管内皮細胞及び(正常)尿路上皮細胞との接着の機構

尿路上皮癌细胞与血管内皮细胞和（正常）尿路上皮细胞之间的粘附机制

• 批准号: 11770885
• 负责人: 藤井 靖久
• 金额: $ 1.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11770885/
• 关键词: 尿路上皮(urithelium); 尿路上皮癌(urothelial cancer); 移行上皮癌(transitional cell carcinoma); 糖鎖抗原(carbohydrate antigen); E-セレクチン(E-selectin); シアリルルイスa(sialyl-Lewis A); シアリルルイスx(sialyl-Lewis X); 接着(adhesion)

1.E-selectinを発現させたヒト臍帯静脈内皮細胞と,ヒト尿路上皮癌細胞との接着実験インターロイキン-1(IL-1)によりE-selectinを発現させた血管内皮細胞と,尿路上皮癌株(JTC-30,JTC-32,T24)との接着をIn vitroで調べた.JTC-30とJTC-32は,E-selectin依存性に血管内皮細胞と接着し,前者では抗Sialyl-lewis^x抗体と抗Sialyl-lewis^a抗体の組み合わせにより,接着はほぼ完全に阻害された.後者の接着は両抗体の併用にて,有意ではあるが軽度の接着の阻害がみとめられるのみであた.T24は,E-selectin依存性の血管内皮細胞との接着を示さなかった.2.尿路上皮癌細胞株の糖鎖抗原Sialyl-lewis^x,Sialyl-lewis^aの発現flow cytometryにより,JTC-30とJTC-32は細胞表面の糖鎖抗原Sialyl-lewis^x,Sialyl-lewis^aの両者を発現しており,T24は何れも発現していなかった.3.臨床材料における糖鎖抗原Sialyl-lewis^x,Sialyl-lewis^aの発現臨床材料として得られた,尿路上皮癌組織を免疫染色した結果,尿路上皮癌の約70%でSialyl-lewis^xかSialyl-lewis^aを発現しており,両者の発現(染色性)は正の相関を示した.また,この染色性と転移率は,統計的に有意な相関をみた.以上1.2.3.の実験により,尿路上皮癌細胞においては,糖鎖抗原Sialyl-lewis^x,Sialyl-lewis^aの両者が,血管内皮細胞との接着性,癌の悪性度,転移性と関係していることが示唆された.また,尿路上皮癌では,両者以外のE-selectin ligandの存在も疑われた.

1.E-selectin expression in umbilical vein endothelial cells, urothelial carcinoma cells, and urothelial carcinoma cells followed by IL-1 expression in vascular endothelial cells, urothelial carcinoma cells (JTC-30,JTC-32,T24) and then In vitro modulation.JTC-30 and JTC-32,E-selectin dependent vascular endothelial cells and then, the former is anti-Sialyl-lewis ^x antibody and anti-Sialyl-lewis ^a antibody combination, then completely blocked. 2. Glycolock Antigen Sialyl-lewis ^x, Sialyl-lewis^a in Urothelial Carcinoma Cell Lines,JTC-30, JTC-32, Sialyl-lewis ^x, Sialyl-lewis ^a in Urothelial Carcinoma Cell Lines 3. Clinical materials were obtained and urothelial carcinoma tissues were immunostained. About 70% of urothelial carcinoma tissues were stained with Sialyl-lewis ^x and Sialyl-lewis ^a. The statistical significance of this correlation is that it is not correlated with the chromatic shift rate. 1.2.3. Urothelial cancer cells are involved in the development of glycogen lock antigens Sialyl-lewis^x,Sialyl-lewis^a, vascular endothelial cells are involved in the development of adhesion, cancer is involved in the development of migration. Urothelial carcinoma is suspected of the presence of E-selectin ligand other than urothelial carcinoma.