High resolution hybrid mass spectrometer

高分辨率混合质谱仪

• 批准号: 420241182
• 金额: --
• 依托单位: Freie Universität Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/420241182?language=en
• 关键词: resolution; hybrid; mass; spectrometer

The goal of this application is the new acquisition of a high resolution mass spectrometer at Freie Universität Berlin. The instrument is planned to be used for proteomic investigations, for the identification of post-translational modifications and for the investigation of intact proteins or protein complexes by native mass spectrometry. As part of the existing BioSupraMol facility the spectrometer is intended to be installed at the Department of Biology/Chemistry/Pharmacy and should serve the diverse requirements of the individual research groups. The intended projects reach from typical protoemic applications for the identification of protein networks in complex mixtures to questions related to structure and molecular mechanism underlying protein complex formation. The biological systems that will be interrogated are also of diverse origin and for example comprise questions of the change of proteomes and interactomes in bacteria upon changing environmental conditions to questions that ask for modifications of proteins upon stimulation of the nervous or immune system.

该应用程序的目标是在自由大学柏林的高分辨率质谱仪的新收购。该仪器计划用于蛋白质组学研究，用于翻译后修饰的鉴定以及通过天然质谱法研究完整蛋白质或蛋白质复合物。作为现有BioSupraMol设施的一部分，光谱仪将安装在生物/化学/药学系，并应满足各个研究小组的不同要求。预期的项目范围从典型的原型应用，用于识别复杂混合物中的蛋白质网络，到与蛋白质复合物形成的结构和分子机制相关的问题。将被询问的生物系统也具有不同的起源，并且例如包括在改变环境条件时细菌中蛋白质组和相互作用组的变化的问题到在刺激神经或免疫系统时要求蛋白质修饰的问题。