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心虚血・再灌流時の心筋細胞拍動リズムゆらぎとダイナミクス

心脏缺血/再灌注期间心肌细胞搏动节律波动和动态

基本信息

批准号：
11780609
负责人：
山内芳子
金额：
$ 1.47万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11780609/
关键词：
虚血・再灌流心筋細胞ゆらぎラット

项目摘要

新生ラット培養心筋細胞を用いて,模擬的虚血・再灌流に伴う拍動リズムゆらぎの変化を明らかにすることを目的として実験を行った.生後1〜3日目の新生ラットの心室からタンパク質分解酵素処理により心筋細胞を単離した.単離した心筋細胞は培養液MCDB107により培養用フラスコ内で5〜10日間培養した.虚血状態の模擬は通常の培養液からグルコースを除去したものに培養液を交換し,さらにフラスコ内にN_2ガスを充満し,閉鎖系とすることで行った.一方再灌流は,フラスコを開放して有酸素化し,通常の培養液に戻すことで模擬した.虚血開始1時間後の時点で拍動周期は有意に延長し,拍動リズムゆらぎの大きさを表す拍動周期の変動係数も有意に増加した.さらに虚血開始3時間後には,ほとんどの細胞が拍動を停止したが,拍動が持続した細胞においても,さらなる拍動周期の延長,及び変動係数の増加が認められた.再灌流後は,一時的に拍動周期の短い,安定した(変動係数の小さい)拍動が出現し,その後1時間までには虚血前の状態に戻った.これ以前の研究において,細胞間の電気的相互作用が培養心筋細胞の拍動リズムゆらぎ大きな影響を与えることがわかっており,虚血負荷中の心筋細胞拍動リズムゆらぎの増加は,虚血状態に伴って起こる細胞内pHの低下により引き起こされる,細胞間の電気的結合の低下によるものである可能性が挙げられる.しかしながら,虚血負荷中の細胞内pHは時間経過に伴って徐々に減少していたが,拍動リズムゆらぎが増大する虚血開始1時間後の時点では,ギャップ結合透過性を十分に減少させるレベルまで低下するには至らなかった.さらに,虚血状態下においても,同一集塊内に存在する心筋細胞の拍動リズムは同期していたことから,本実験で用いた虚血負荷により誘発される拍動リズムゆらぎの増加は,細胞間の電気的相互作用の減少に依るものではなく,培養心筋細胞の興奮特性の変化によるものである可能性が示唆された.

The newborn baby trains the heart to use the drug, and the virtual blood of the model is reperfused with the blood of the model, which is reperfused with the blood of the model. On the 1st and 3rd day after birth, the newborn was born, the ventricle was broken down, the enzymes were broken down, and the heart cells were isolated. Stay away from the heart muscle cell culture solution and use the MCDB107 cell culture solution within 5 ~ 10 days. The model of deficiency blood state is usually caused by the removal of the liquid, the fluid, the liquid, the liquid, the liquid When one side is reperfused, there is acidification on the other side, and the culture solution is usually used to treat the mold. At any time after the start of the virtual blood, the activity cycle is intentionally prolonged, and the number of activity cycles is intentionally increased. Three hours after the start of the depletion of blood, the cell beat stops, the cell cycle lengthens, and the number of cycles increases. After reperfusion, the beat cycle was short, diazepam was detected, and the state was abnormal before deficiency blood was detected 1 time later. In the past, we have studied the interaction between cells and computers, and the interaction between cells and computers has been studied in the past, and the interaction between cells and computers has been studied in the past, the interaction between cells and computers has been studied in recent years, and the interaction between cells and computers has been studied in the past, and the interaction between cells has been studied in the past, and the interaction between cells has been developed. The combination of intercellular electricity is low. The possibility of failure is low. During the incubation period, the intracellular pH time of hypotension and hypotension is very low. During this period of time, you can slowly reduce the amount of blood loss in the cell. After the start of the first hour of hypotension, the depletion of blood will start at 1: 00 p. M. in combination with very low levels of blood pressure. In the same episode, there is a change of heart and heart in the same episode. In the same episode, there is a significant increase in the number of cells in the same episode. In the same episode, there is a significant increase in the interaction between cells. Cultivate your mind, your cells, your characteristics, your possibilities, your possibilities.