High resolution hybrid mass spectrometer with ion chromatography

代谢物是基因表达和物质进出生物体之间相互作用的结果。它们与蛋白质一起定义了有机体的表型。代谢组学与其他组学水平的技术一起构成了系统生物学的支柱之一。代谢组学与全基因组关联研究、基因敲除或基因错误表达等遗传学方法相结合，对于目前未知基因的识别和功能关联以及对新陈代谢调控的理解都是至关重要的。代谢组学需要对小分子进行全面的高通量分析。色谱分离与质谱学检测相结合，能够对复杂的分析物进行常规和全面的定量和定性分析，如植物或动物组织中包含的代谢物。海因里希·海涅大学杜塞尔多夫分校目前没有建立起对带电和高极性代谢物的代谢组学分析，如Calvin-Benson循环或三羧酸循环的中间产物糖酵解。这项提议为购买高分辨率离子色谱/质谱学系统以同时对代谢物进行定量和定性分析奠定了基础。

Metabolites are the result of the interaction between gene expression and the flux of substances into and out of a living organism. They define, together with proteins, the phenotype of an organism. Jointly with other omics-level technologies, metabolomics constitutes one of the pillars of systems biology. In combination with genetic approaches, such as genome-wide association studies, gene knockouts, or mis-expression of genes, metabolomics is crucial for the identification and functional association of to-date unknown genes, and for understanding the regulation of metabolism. Metabolomics requires the comprehensive high-throughput analysis of small molecules. Chromatographic separation hyphenated with mass spectrometric detection enables the routine and comprehensive quantitative and qualitative analysis of complex analytes, such as the metabolites contained in plant or animal tissues. Heinrich Heine University Düsseldorf is currently not set up for the metabolomic analysis of charged and highly polar metabolites, such as intermediates glycolysis, of the Calvin-Benson cycle, or the tricarboxylic acid cycle. This proposal is building the case for the purchase of a high-resolution ion chromatography/mass spectrometry system for the simultaneous quantitative and qualitative analysis of metabolites.