神経管閉鎖不全症の転帰と無脳症病態成立機序の解明

阐明神经管缺陷的结果和无脑畸形的发病机制

• 批准号: 12770602
• 负责人: 八田 稔久
• 金额: $ 1.22万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12770602/
• 关键词: 外脳症; 無脳症; 子宮外発生法; Peeled off mode; Resorbed mode; 5-azacytidine; マウス; 胎児; 神経管閉鎖不全; 中枢神経奇形

無脳症および外脳症は、胎生期における神経管閉鎖の障害に起因する中枢神経系の発生異常と考えられている。これまでに多数のヒト外脳症胎児における観察と、動物を用いた実験奇形学的な研究から、露出脳組織が在胎期間中に表面から徐々に脱落して無脳症に移行すると類推されてきた。しかしながら、外脳症と無脳症の直接的な因果関係を含む、外脳症の転帰についての詳細な研究は行われていない。我々は、5-azacytidineにより誘発したマウス外脳症胎児をモデルとして用いた。ネンブタール麻酔下に、胎齢13.5日に、子宮切開を施し外脳症胎児を顕微鏡下に直接観察を行った。その後、子宮外発生法を用いて発生を継続させ、胎齢18.5日に再び観察し、外脳症の転帰について同一個体において検討を行った。その結果、胎齢13.5日に認められた外脳症全例が無脳症へ移行するわけではなく、様々な外脳組織の形態変化を示す固体(ばらつき)が認められた。この移行パターンを分類化し、更に組織学的検討を行った所、間脳・中脳由来の外脳組織の脱落機構として、皮が剥がれるように外脳組織が脱落する"Peeled off"mode、および神経管の閉鎖には異常のない大脳において組織が脳底側から吸収されるように脱落する"Resorbed"mode、という二つの組織脱落様式の存在を提唱に至った。また、色素注入による脳血管支配の詳細な観察を行った所、外脳症胎仔の頭部主要血管の走行・分布に、外脳症の形態により大きな変異を認め、末梢循環不全や出血を介した上記の組織脱落様式への関与が示唆された。これらの結果より、外脳症の転帰における複雑な移行パターンは、上記二つの組織脱落様式の組み合わせにより説明できるのではないかと考えられた。

Asymptomatic eclampsia, prenatal eclampsia, fetal eczema, postnatal eczema, postnatal eclampsia, postpartum eczema, postnatal eclampsia, postpartum eclampsia, postpartum eczema, postnatal eczema, postnatal eclampsia. Most of the parturients with exotropia were examined, and the animal was used to study the singularity of biomedicine, which revealed that the tissue tissue had no symptoms during the fetal period and was transferred to the placenta. The cause and effect of the disease is not affected. The cause and effect of the disease is not affected. The study of the disease. I would like to know if you are suffering from an exopathic fetus. I would like to know if you are using 5-azacytidine. The fetus was cut for 13.5 days, and the fetus was cut open for the treatment of exopathic fetus. After the birth of the baby, the external hygiene method was used to give birth to the baby, the fetus was examined again after 18.5 days, and the external symptoms were detected in the same body. The results showed that the whole case of 13.5-day pregnancy without symptoms was transferred to normal tissue, and the morphology of external tissue showed that it was solid. You need to learn how to classify your home, change your organization, make sure that your tissue falls off, that your skin is peeled off, that your tissue falls off, that you Peeled off your mode, that you learn how to use it, and that you learn how to use it to make sure that you don't know how to use it. You need to learn how to do it. You need to know how to do it. You can use it to make sure that you don't know how to do it. It's called "Resorbed" mode. There is a way to raise the sound of tissue shedding in the second place. The distribution of the main blood vessels in the fetus with exopathia was observed, the main blood vessels in the fetus with exopathia were observed, and the blood vessels were detected by blood vessel instillation, pigment injection, vascular innervation, peripheral bleeding, tissue exfoliation and instigation. The results of this study are as follows: the results of this study, the results of this study, the results of the test, the results of the results, the results of the study, the results of the results, the results of the disease, the results of the ex

项目成果

Akihiro Masumoto: "Sequential observation of exencephaly and subsequent morphological changes by mouse exo utero development system. -Analysis of the mechanism of transformation from exencephaly to anaencephaly-"Anatomy and Embryology. (in press). (2002)

Akihiro Masumoto：“小鼠子宫外发育系统对外脑畸形及其后续形态变化的顺序观察。-从外脑畸形到无脑畸形转化的机制分析-”解剖学与胚胎学。