Functional dissection of transport processes during glycopeptide antibiotic production (B02)

细菌生物合成天然化合物（如抗生素）的基因簇主要编码所产生的化合物的出口。balhimycamycolatopsis balhimycina是糖肽抗生素balhimycin的生产者，编码ABC出口Tba，这对balhimycin的分泌至关重要，但对产生菌株的自我抗性无关。在这里，我们想进一步表征Tba在balhimycin生产中的作用，并评估其对糖肽抗生素的特异性。我们将采用综合的方法，包括细菌遗传学、次级代谢物的靶向分析、蛋白质定位、蛋白质-蛋白质相互作用分析以及体外转运分析。

Bacterial biosynthetic gene clusters of natural compounds – like antibiotics – mostly encode exporters of the compounds produced. Amycolatopsis balhimycina, producer of the glycopeptide antibiotic balhimycin, encodes the ABC exporter Tba, which was shown to be critical for balhimycin secretion but not for self-resistance of the producer strain. Here, we want to further characterize the role of Tba in balhimycin production and assess its specificity towards glycopeptide antibiotics. We will employ an integrated approach using bacterial genetics, targeted analysis of secondary metabolites, protein localization, and protein-protein interaction analysis as well as in vitro transport assays.