Functional dissection of transport processes during glycopeptide antibiotic production (B02)

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Bacterial biosynthetic gene clusters of natural compounds – like antibiotics – mostly encode exporters of the compounds produced. Amycolatopsis balhimycina, producer of the glycopeptide antibiotic balhimycin, encodes the ABC exporter Tba, which was shown to be critical for balhimycin secretion but not for self-resistance of the producer strain. Here, we want to further characterize the role of Tba in balhimycin production and assess its specificity towards glycopeptide antibiotics. We will employ an integrated approach using bacterial genetics, targeted analysis of secondary metabolites, protein localization, and protein-protein interaction analysis as well as in vitro transport assays.